PURPOSE: The main purpose of this study was to investigate the protective role of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. METHODS: Human erythrocyte membrane bound Ca(2+)-ATPase and Na(+)/K(+)-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-gallate was evaluated by in vitro experiments by adding epigallocatechin-3-gallate in concentration dependent manner (final concentration range 10(-7)M to 10(-4)M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10(-5) M final concentration). RESULTS: We have reported concentration dependent effect of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced damage on activities of Ca(2+)-ATPase and Na(+)/K(+)-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca(2+)-ATPase and Na(+)/K(+) -ATPase as a function of human age. Epigallocatechin-3-gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. CONCLUSION: Epigallocatechin-3-gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca(2+)-ATPase and Na(+)/K(+) -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
PURPOSE: The main purpose of this study was to investigate the protective role of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. METHODS:Human erythrocyte membrane bound Ca(2+)-ATPase and Na(+)/K(+)-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-gallate was evaluated by in vitro experiments by adding epigallocatechin-3-gallate in concentration dependent manner (final concentration range 10(-7)M to 10(-4)M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10(-5) M final concentration). RESULTS: We have reported concentration dependent effect of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced damage on activities of Ca(2+)-ATPase and Na(+)/K(+)-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca(2+)-ATPase and Na(+)/K(+) -ATPase as a function of human age. Epigallocatechin-3-gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. CONCLUSION:Epigallocatechin-3-gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca(2+)-ATPase and Na(+)/K(+) -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
Authors: Antonio Bustos; Pablo Selvi Sabater; María Benlloch; Eraci Drehmer; María Mar López-Rodríguez; Felix Platero; Jose Luis Platero; Jesús Escribá-Alepuz; Jose Enrique de la Rubia Ortí Journal: Medicina (Kaunas) Date: 2021-01-24 Impact factor: 2.430