Xudong Wei1, Jingyu Wang2, Jian He1, Bingjuan Ma1, Jing Chen1. 1. Department of Otolaryngology-Head and Neck Surgery, Gansu Provincial Hospital Lanzhou 730000, China. 2. Institute of Pathophysiology, Lanzhou University Lanzhou 730000, China.
Abstract
OBJECTIVE: To investigate the in vitro invasive capability, clone-forming ability, resistance to anti-tumor treatments of CD133(+) human laryngeal carcinoma stem cells, and characterize the related signaling pathways in these cells. METHODS: Human laryngeal carcinoma Hep-2 cells were subjected to flow cytometry sorting to obtain CD133(+) stem cells. Transwell chamber assay and clone-formation forming test were performed to evaluate the invasive capability and the clone-forming ability of CD133(+) laryngeal carcinoma tumor stem cells, respectively. MTT assay was used to assess the resistance of CD133(+) Hep-2 cells to radiotherapy and chemotherapy, respectively. Western blot and real-time PCR were applied to characterize the signaling pathways in these stem cells. RESULTS: Our results from the transwell chamber assay indicated that the migrating capability of CD133(+) Hep-2 cells was significantly higher than CD133(-) cells, and the invasive capability of CD133(+) Hep-2 cells was also significantly elevated. Moreover, clone-formation forming test showed higher clone-forming ability for CD133(+) Hep-2 cells, compared with CD133(-) cells. Furthermore, CD133(+) Hep-2 cells displayed significant resistance to radiotherapy and chemotherapy. The Bcl-2/Bax ratio was increased, and Hedgehog, Wnt, and Bmi-l signaling pathways were all activated, in CD133(+) laryngeal carcinoma stem cells, which might be involved in the self-renewal process of these stem cells. CONCLUSION: The invasive capability, clone-forming ability, and resistance to anti-tumor treatments are enhanced, and anti-apoptotic and proliferation-related signaling pathways are activated in CD133(+) laryngeal carcinoma tumor stem cells. These findings might provide new insights into the prevention and/or treatment of laryngeal carcinoma, especially concerning target-oriented therapies.
OBJECTIVE: To investigate the in vitro invasive capability, clone-forming ability, resistance to anti-tumor treatments of CD133(+) humanlaryngeal carcinoma stem cells, and characterize the related signaling pathways in these cells. METHODS:Humanlaryngeal carcinoma Hep-2 cells were subjected to flow cytometry sorting to obtain CD133(+) stem cells. Transwell chamber assay and clone-formation forming test were performed to evaluate the invasive capability and the clone-forming ability of CD133(+) laryngeal carcinomatumor stem cells, respectively. MTT assay was used to assess the resistance of CD133(+) Hep-2 cells to radiotherapy and chemotherapy, respectively. Western blot and real-time PCR were applied to characterize the signaling pathways in these stem cells. RESULTS: Our results from the transwell chamber assay indicated that the migrating capability of CD133(+) Hep-2 cells was significantly higher than CD133(-) cells, and the invasive capability of CD133(+) Hep-2 cells was also significantly elevated. Moreover, clone-formation forming test showed higher clone-forming ability for CD133(+) Hep-2 cells, compared with CD133(-) cells. Furthermore, CD133(+) Hep-2 cells displayed significant resistance to radiotherapy and chemotherapy. The Bcl-2/Bax ratio was increased, and Hedgehog, Wnt, and Bmi-l signaling pathways were all activated, in CD133(+) laryngeal carcinoma stem cells, which might be involved in the self-renewal process of these stem cells. CONCLUSION: The invasive capability, clone-forming ability, and resistance to anti-tumor treatments are enhanced, and anti-apoptotic and proliferation-related signaling pathways are activated in CD133(+) laryngeal carcinomatumor stem cells. These findings might provide new insights into the prevention and/or treatment of laryngeal carcinoma, especially concerning target-oriented therapies.
Authors: M E Prince; R Sivanandan; A Kaczorowski; G T Wolf; M J Kaplan; P Dalerba; I L Weissman; M F Clarke; L E Ailles Journal: Proc Natl Acad Sci U S A Date: 2007-01-08 Impact factor: 11.205
Authors: Piero Dalerba; Scott J Dylla; In-Kyung Park; Rui Liu; Xinhao Wang; Robert W Cho; Timothy Hoey; Austin Gurney; Emina H Huang; Diane M Simeone; Andrew A Shelton; Giorgio Parmiani; Chiara Castelli; Michael F Clarke Journal: Proc Natl Acad Sci U S A Date: 2007-06-04 Impact factor: 11.205
Authors: Tomasz Kolenda; Weronika Przybyła; Marta Kapałczyńska; Anna Teresiak; Maria Zajączkowska; Renata Bliźniak; Katarzyna M Lamperska Journal: Rep Pract Oncol Radiother Date: 2018-03-17
Authors: A Greco; Maria Ida Rizzo; A De Virgilio; A Gallo; M Fusconi; G Pagliuca; S Martellucci; R Turchetta; M De Vincentiis Journal: Eur Arch Otorhinolaryngol Date: 2015-11-19 Impact factor: 2.503