Laparoscopic radical prostatectomy with bladder neck preservation: positive surgical margin and urinary continence status.

INTRODUCTION: Preservation of the bladder neck (BN) has been controversial, as limited excision of the bladder neck may result in incomplete resection of the disease. Moreover, the urinary continence rate may not be improved. AIM: To evaluate the effect of bladder neck sparing on urinary continence, and surgical margins status in prostate cancer (PCa) patients treated with laparoscopic radical extraperitoneal prostatectomy.
MATERIAL AND METHODS: A retrospective analysis of 295 consecutive patients who had undergone laparoscopic radical extraperitoneal prostatectomy for clinically localised prostate cancer in a single institution was performed. Positive surgical margin (SM(+)) and urinary continence status at 3, 6, and 12 months were evaluated.
RESULTS: The distribution of SM(+) for pT2, pT3, and pT4a was 15.3% (27/176), 49.1% (58/118), and 100% (1/1), respectively. Overall, there were 55.61%, 80.61%, and 84.69% of men continent at 3, 6, and 12 months, respectively. However, when limiting the analysis to those who did not receive adjuvant radiotherapy within 12 months following surgery, urinary continence rates were 59.23%, 85.86%, and 90.21% at 3, 6, and 12 months, respectively. Preoperative prostate-specific antigen (PSA) and pathological T stage were identified as significant predictors of positive surgical margins.
CONCLUSIONS: Laparoscopic radical extraperitoneal prostatectomy with bladder neck preservation has been a safe procedure which has resulted in good functional outcome. We observed a relatively high incidence of positive surgical margins which could be attributed to a large number of extracapsular disease cases.

Introduction

Radical prostatectomy is considered the “gold standard” treatment for patients with localised prostate cancer and life expectancy greater than 10 years [1]. Regardless of the technique of the procedure being performed, its primary goal remains to achieve excellent cancer control. However, this has to be balanced against the morbidities including urinary incontinence and quality of life [1-5]. With the intent of reducing the invasiveness of an open approach, in 1991 Schuessler et al. performed the first laparoscopic prostatectomy [6]. Since then the shift from open to endoscopic surgery has begun, as the minimally invasive techniques offer magnification of the operative field and enable more precise identification and dissection of the anatomical structures [7-9]. However, even with the advancement of laparoscopic and robotic surgery, the likelihood of post-prostatectomy urinary incontinence may be high and ranges, depending on the definition of urinary control, from 2.5% to 87% [10]. To improve the functional outcomes of the surgery, several different centres across the world have developed more refined techniques which allow for nerve sparing, apical dissection and bladder neck preservation (BNP) [7, 11, 12]. However, BNP remains controversial, as it may come at the cost of high risk of positive surgical margins, compromising the disease prognosis, and not necessarily being able to guarantee a better continence outcome [13, 14].

Aim

Therefore, the aim of this study is to evaluate the effect of bladder neck sparing on urinary continence and surgical margins status in prostate cancer patients treated with laparoscopic radical extraperitoneal prostatectomy.

Material and methods

Clinical and histological data of 295 consecutive patients, who had undergone laparoscopic radical extraperitoneal prostatectomy (LRP) for clinically localised prostate cancer in a single institution, between January 2007 and December 2012, were reviewed from a prospectively maintained database. All of the procedures were performed by two experienced surgeons. Bladder neck preservation technique was applied with a bladder neck circumference approximating the urethral stump before anastomosis, as previously described [7, 8]. As BN sparing was performed consistently, we did not exclude patients with median lobe hypertrophy or those with high-risk features from this bladder neck dissection technique. All pathological examinations were performed by a single pathologist. A positive surgical margin was defined as the presence of cancer cells at the inked margin of the resected specimen.

Perioperative parameters such as operative time, transfusion and conversion rates, catheterisation time, pathological results and positive surgical margin rates were reviewed from our prospectively maintained database. Urinary continence status was assessed with the ICIQ-UI Short Form questionnaire at 3, 6 and 12 months after every laparoscopic radical prostatectomy [15]. Postoperative continence was defined as the absence of need for any pads. Patients who had less than 12 months of follow-up were excluded from the postoperative continence status analysis. A total of 196 men met these inclusion criteria and were analysed respectively. Follow-up comprised clinical examination and evaluation of the PSA levels at 3, 6, 9 and 12 months after laparoscopic radical prostatectomy and every 6 months thereafter. Biochemical recurrence (BCR) was defined as two consecutive PSA levels of > 0.2 ng/ml after LRP.

Statistical analysis

The Shapiro-Wilk test was used to examine for normal distribution. Results were presented as mean values ± standard deviation (± SD) when data were normally distributed, otherwise as median, 1st and 3rd quartile (Q1, Q3). Parametric t test and Mann-Whitney U tests were applied as appropriate. The associations between parameters were examined using Spearman's rank correlation coefficient. Kaplan-Meier method and the log rank test were used for biochemical recurrence-free survival analyses. For all statistical tests a p value < 0.05 was considered statistically significant. The Statistical Package for the Social Sciences (SPSS), version 19, was used for all statistical analyses.

Results

Patient demographic data are presented in Table I and intraoperative data in Table II. The mean patient age was 62 years (range: 42–78 years). The most common preoperative Gleason score was 6. Mean operative time (from skin incision to skin closure) was 156 min (range: 85–370 min). The total perioperative transfusion rate was 3.7%, and the intraoperative transfusion rate was 1.3% (4 patients required blood transfusion during surgery). Five patients required conversion to an open procedure: one occurred in an obese man due to the lack of extra-long instruments and the inability to perform anastomotic suturing; two others were due to suboptimal vision due to camera malfunction; and two cases were converted to open surgery due to problems with the gas insufflator and the inability to maintain adequate intra-abdominal pressure.

Table I

Patient demographics

Variable	Value
Age, mean ± SD [years]	62 ±6.40
PSA, mean ± SD [ng/ml]	10.38 ±12.21
Median Gleason score	6
Preoperative Gleason score [%]:
4	9
5	16
6	47
7	22
8	3
9	3

SD – standard deviation

Table II

Intraoperative data

Variable	Value
Operative time, mean ± SD [min]	156 ±47
Transfusion rate (%)	1.3
Conversion rate (%)	1.6
LOS, mean ± SD [days]	8.05 ±2.80
Catheterisation time, mean ± SD [days]	19.23 ±5.68

LOS – length of hospital stay

There were no intraoperative complications. The mean hospital stay was 8 days (range: 2–14 days) and the mean catheter time was 19 days (range: 9–36 days). The median follow-up was 36 months (range: 12–60 months).

Oncologic results are presented in Table III. The most common Gleason score determined postoperatively was 6. The distribution of pathologic stages was 59.7% pT2, 40.0% pT3 and 0.3% pT4. The overall positive surgical margin rate was 29.15% (86 cases). Of those, 30.2% (27/86) were observed in men with pT2 stage of prostate cancer, 67.4% (58/86) were detected in patients with pT3 stage, and 1.16% (1/86) of SM(+) was noted in a man with pT4a disease. The distribution of SM(+) for pT2, pT3, and pT4a was 15.3% (27/176), 49.1% (58/118), and 100% (1/1), respectively.

Table III

Oncologic results

Variable	Value
pT2a, n (%)	40 (13.6)
pT2b, n (%)	15 (5.1)
pT2c, n (%)	121 (41.0)
pT3a, n (%)	96 (32.0)
pT3b, n (%)	22 (7.5)
pT4a, n (%)	1 (0.3)
Median Gleason score	6
Postoperative Gleason score (%):
4	5.1
5	18.9
6	38.3
7	30.2
8	4.1
9	3.4
Overall SM(+), n (%)	86 (29.15)
SM(+) by stage:
pT2a, n (%)	2 (5.0)
pT2b, n (%)	4 (26.6)
pT2c, n (%)	21 (17.3)
pT3a, n (%)	44 (45.8)
pT3b, n (%)	14 (63.6)
pT4a, n (%)	1 (100)

SM(+) – positive surgical margin

Overall, 59 patients (20.0%) had an isolated SM(+) and 27 men (13.7%) had multiple positive margins. Of those, 29.0% of patients (25/86) had a solitary apical SM(+), whereas an isolated positive surgical margin was present in posterolateral, posterior, lateral and anterior location in 16.3% (14/86), 12.8% (11), 4.7% (4/86), and 3.5% (3/86) of cases, respectively.

The bladder neck was a positive margin in 14 instances (16.3%), and in 12 out of those cases (85.7%) it was in combination with an SM(+) at 1 or 2 other sites (apex in 6 men, anterior in 4 cases, and posterolateral in 4 patients).

Postoperative urinary continence status was assessed in 196 patients at 3, 6 and 12 months after surgery (Table IV). Overall, there were 55.61%, 80.61%, and 84.69% of men continent, respectively. However, when limiting the analysis to those who did not receive adjuvant radiotherapy within 12 months following surgery (184 men), urinary continence rates were 59.23%, 85.86%, and 90.21% at 3, 6, and 12 months, respectively.

Table IV

Postoperative continence rates with (RXT+) and without adjuvant radiotherapy (RXT–)

Postoperative time [months]	Continence rate (RXT+) (%)	Continence rate (RXT–) (%)
3	55.61	59.23
6	80.61	85.86
12	84.69	90.21

Continence rate (RXT+) – continence rates of all patients including those who underwent adjuvant radiotherapy, continence rate (RXT–) – continence rates of patients who did not receive adjuvant radiotherapy

Overall, detectable prostate-specific antigen levels of > 0.20 ng/ml were found in 14 patients (7.1%). Six cases were detected in patients with negative surgical margins (SM(–)) (2.8% of the SM(–) group), and 8 men had positive SM (9.3% of the SM(+) group). The mean time to detectable PSA recurrence was 22.05 months in patients with negative surgical margins and 18.17 months in men with positive surgical margins, p = 0.182. The overall biochemical recurrence-free survival rate was 74.2% (141/190) at 3 years.

Four patients developed bone metastases. Of those, 3 had SM(–) and 1 had SM(+). The median time to a positive bone scan was 40 months.

Adjuvant or salvage radiation treatment (RXT) was provided to 57 men during 3 years of follow-up. Of those, 12 underwent radiotherapy during the first 12 months of follow-up, 17 men had RXT between 12 and 24 months following surgery, and 28 patients received radiation treatment in the third year of follow-up. For patients with presumed local recurrence (2 cases) who refused RXT, expectant management was offered. Men with a systematic relapse were managed with androgen deprivation treatment.

The surgical margin status was significantly correlated with the preoperative PSA (p = 0.008), clinical T stage (p = 0.006), and pathological T stage (p < 0.001). Patients with positive surgical margins more commonly had a lesion staged as cT2b and, less frequently, a tumour determined as cT2a on digital rectal examination than men with negative surgical margins (15.1% and 20.9% vs. 33.0% and 11.0%, respectively). Moreover, a higher pathological T stage of prostate cancer (pT3a and pT3b) was observed more frequently in men with SM(+) compared to those with SM(–) (51.1% and 16.3% vs. 24.9% and 4.3%, respectively). Preoperative median PSA level in patients with negative surgical margins was lower than in cases with SM(+) (7.70 ng/ml (5.55; 10.80) vs. 8.38 ng/ml (6.87; 11.81), respectively).

There was no statistical difference in the age of patients when analysed by the status of surgical margins (61.85 ±6.08 years in men with negative SM, and 62.79 ±7.07 years in men with SM(+), p = 0.249). Similarly, there was no statistical difference observed in the median biopsy and postoperative Gleason score when analysed by the SM status (the median biopsy and postoperative Gleason score in both groups of men was 6, p = 0.639, and p = 0.071, respectively). To further evaluate a possible association of Gleason score with surgical margin status, we also analysed outcomes by Gleason score into low, intermediate or high grades based on the Gleason score of 4–6, 7, and 8–10, respectively. This analysis identified no statistical difference in the frequency of those three biopsy and postoperative grades in men with SM(+) and SM(–) (p = 0.450, and p = 0.162, respectively).

The logistic regression analysis indicated that preoperative PSA, and pathological T stage correlated with positive surgical margin (p = 0.008, r = 0.154, and p < 0.001, r = 0.371, respectively). There was no correlation between surgical margin status and the biopsy and postoperative Gleason score, or clinical T stage.

Surgical margin status had a significant effect on the 3-year biochemical recurrence-free survival, with a higher percentage of men without PSA recurrence in those with negative surgical margins (89.9% vs. 55.8%, respectively, p < 0.001) (Figure 1).

Figure 1

Kaplan-Meier curve showing the biochemical recurrence-free survival rates (progression PSA greater than 0.2 ng/ml) according to surgical margin status

BCR-free survival – biochemical recurrence-free survival; SM(+) – positive surgical margin, SM(–) – negative surgical margin, PSA – prostate-specific antigen

To evaluate the prognostic value of preoperative PSA, we divided the patients with and without positive surgical margins into those with PSA ≤ 10 ng/ml, and > 10 ng/ml. Men with PSA > 10 ng/ml had significantly shorter time to biochemical recurrence (83.3% vs. 92.3% in cases with SM(–), respectively, p = 0.047, and 39.2% versus 65.4% in patients with SM(+), respectively, p = 0.027) (Figures 2 and 3).

Figure 2

Kaplan-Meier curve showing biochemical recurrence-free survival rates (progression PSA greater than 0.2 ng/ml) according to preoperative PSA strata in men with negative surgical margin

BCR-free survival – biochemical recurrence-free survival, PSA – prostate-specific antigen

Figure 3

Kaplan-Meier curve showing biochemical recurrence-free survival rates (progression PSA greater than 0.2 ng/ml) according to preoperative PSA strata in men with positive surgical margin

BCR-free survival – biochemical recurrence-free survival, PSA – prostate- specific antigen

Discussion

Urinary continence in men depends on the coordinated function of two independent sphincter mechanisms, which are the proximal urethral sphincter, located within the bladder neck, and the distal urethral sphincter, anchored below the verumontanum [16, 17]. Injury to either one can result in the loss of urinary control [18, 19].

Radical prostatectomy, which most commonly leads to bladder neck dysfunction, may also cause intraoperative damage of the neuronal innervation, which itself can adversely affect the continence mechanisms [18, 20]. In order to preserve these critical structures, and consequently improve functional outcome, various technical modifications have been introduced, including neurovascular bundle and bladder neck preservation [7, 11, 12]. However, despite BNP during radical prostatectomy being performed for more than 20 years, it still remains unclear how this technical refinement affects urinary continence, particularly in the long term, but more importantly whether or not it carries a risk for the positive surgical margins [10, 11, 21].

In our study, overall urinary continence rates at 3, 6, and 12 months were 55.61%, 80.61%, and 84.69%, respectively. However, this analysis included data from incontinent men who had adjuvant radiotherapy within 12 months of surgery. To eliminate a potential bias, associated with an adverse radiotherapeutic effect on the continence mechanisms, we excluded those patients from the analysis. The resultant data showed greater continence rates at all 3 points of assessment (59.23%, 85.86%, and 90.21% at 3, 6, and 12 months, respectively). These results are comparable with current literature [4, 7, 22, 23].

Bladder neck preservation carries a potential risk for positive surgical margins as limited excision of the bladder neck may result in the incomplete resection of the disease. In our study, the overall SM(+) rate was 29.15%. The distribution of SM(+) for pT2, pT3, and pT4a was 15.3% (27/176), 49.1% (58/118), and 100% (1/1), respectively.

Several previous studies have addressed the risk of SM(+) after LRP. A positive surgical margin rate of 16.6% to 39.4% has been reported [24, 25]. In studies evaluating the effect of bladder neck preservation on tumour resection, the overall SM(+) rate was up to 32% [25]. A review of 555 radical prostatectomies found the presence of positive surgical margins in 32% of patients [26]. In that study 54% of men with extracapsular extension of the disease had SM(+). Deliveliotis et al. reported a 21% incidence of SM(+) in patients who underwent radical prostatectomy with a BNP procedure, whereas Poon et al. described a 27.4% positive surgical margin rate with bladder neck preservation [27, 28]. Similarly, a recent report from the U.K., which evaluated the oncological results in 575 patients who underwent endoscopic extraperitoneal radical prostatectomy, identified 135 men (23.5%) with positive surgical margins [29]. The SM(+) rate for pT2 disease was 66/406 patients (16.3%) and for pT3 disease it was 68/168 patients (40.5%). Terakawa et al. reported an overall positive surgical margin rate of 39.4%, with a rate of 30.2% found in patients with pT2 disease and 71.0% in patients with pT3 disease [25].

However, several studies evaluating the oncological outcome of laparoscopic and robotic radical prostatectomies found lower rates of SM(+) [5, 7, 22, 30, 31]. The review of 1,564 cases from the Memorial Sloan-Kettering Cancer Center in the U.S.A. reported that only 13% of patients who underwent laparoscopic prostatectomy had positive surgical margins [30]. Similar results were observed in research from Harvard [31]. In a recent study of 95 men who underwent robot-assisted laparoscopic radical prostatectomy with BNP, the overall SM(+) rate was 14.7% [22]. Generally, in more recent reports, the observed better oncological outcome can likely be attributed to the better surgical technique, and the earlier tumour detection, but also to the low rate of extracapsular disease [7]. In our study, the overall positive surgical margin rate was relatively high. This is most likely associated with at least twice greater frequency of extracapsular disease present in our patients (40.33%) than reported in other series with lower SM(+) rates (4.5–27%) [5, 7, 30–32]. It should be emphasized that the use of radical prostatectomy for high-risk PCa and the use of minimally invasive treatment alternatives for low-risk disease have become more popular in many countries in Europe and the U.S.A. over the last decade [33, 34]. It is likely that this trend will continue, and the patients with very low-risk disease or men older than 70 years who are candidates for active surveillance will select minimally invasive surgery, whereas in the high-risk group, indications for radical prostatectomy may expand, because this surgery with extended pelvic lymph node dissection might improve the oncological outcome [35]. Further polarization of radical prostatectomy into a minimally invasive procedure with the goal of preserving function or extensively radical prostatectomy aiming to achieve extensive resection will possibly become more distinct in future.

In our study, we demonstrated that the anatomic dissection with preservation of the bladder neck is an oncologically safe procedure. Although there were 14 patients with a positive bladder neck margin, the great majority (12/14) had SM(+) at multiple sites, indicating that a positive surgical margin would have occurred regardless of bladder neck preservation.

In the present study, factors correlating with cancer aggressiveness (i.e. preoperative PSA and pathological T stage) were independently associated with an increased risk of SM(+). Whereas some previous groups have also identified those two factors as predictors of positive margins, others did not confirm any correlation with SM(+) and indicated the biopsy Gleason score, clinical T stage, prostate volume, interfascial neurovascular bundle dissection and body mass index to be independent predictors of SM(+) [22, 25, 36, 37].

In this study the presence of positive surgical margins significantly affected the 3-year biochemical recurrence-free survival rate (55.8% vs. 89.9% in SM(+) and SM(–), respectively, p < 0.001). The effect of positive surgical margins status on 3-year biochemical recurrence-free survival rates was particularly pronounced in those with preoperative PSA > 10 ng/ml (39.2% vs. 65.4% in men with PSA ≤ 10 ng/ml, p = 0.027). Although there were no such analyses performed in previously reported studies describing results of laparoscopic radical prostatectomy with bladder neck preservation, the importance of the positive surgical margins and preoperative PSA level on the risk of biochemical recurrence has been well recognised [38-40].

It is well known that SM(+)s significantly increase the risk of BCR. However, there is a lack of association between positive margins and cancer-specific mortality (CSM), which is likely due to the highly variable natural history of biochemical recurrence. The data evaluating the risk of metastatic progression and death in men with SM(+) and BCR have stressed the importance of other adverse clinical and pathological parameters than just the presence of positive margins alone, as absence of those features in men with SM(+)s may be associated with an indolent form of BCR [41]. In a recent, large population-based study using the Surveillance Epidemiology and End Results (SEER) cancer registry, in which patients were followed for a median of 4.2 years, a 1.7-fold increased risk of death from PCa was observed among men with positive compared with negative surgical margins [42]. However, after adjustments for adverse pathological features, these findings held true for only high-grade tumours or extraprostatic disease (pT3). Another study by Chalfin et al. showed that an SM(+) was an independent predictor of prostate-cancer-specific mortality (hazard ratio (HR) was 1.4), but the impact was of a modest effect compared to the Gleason score (HR = 5.7–12.6) and pathological stage (HR = 2.2–11.0) [43]. Several other studies have failed to identify SM(+)s to be associated with an increased risk of systemic progression and cancer-specific mortality [44-46]. Similarly, the most recent report with the longest follow-up indicated that SM(+)s alone were not associated with a significantly increased risk of CSM within 15 years [47]. In this study men with SM(+) had 1.04-fold risk of death from PCa compared to those with negative surgical margins.

For the time being, it remains unclear why positive surgical margins may affect probability of BCR but not distant metastasis or cancer-specific mortality, particularly as SM(+)s increase the risk of disease recurrence [48]. It is likely that competing causes of mortality may obscure the detrimental impact of SM(+)s on death due to PCa [46, 49]. However, urologists should strive to avoid positive surgical margins, as they increase the risk of BCR and consequently the need for secondary treatment and may be a source of anxiety among affected patients [50, 51].

The present study has some limitations. Our research is retrospective and it is, therefore, subject to bias and limitations frequently associated with this type of study. Moreover, we have not analysed the effect of size of the prostate, and the presence of a large middle lobe on the functional outcomes. In addition, median follow-up was relatively short (3 years), and consequently the sample size analysed at 12, 24 and 36 months of follow-up was relatively small. Additionally, data regarding postoperative urinary continence at 12 months of follow-up were available in 196 out of 295 patients who underwent the surgery.

Conclusions

Laparoscopic radical extraperitoneal prostatectomy with bladder neck preservation proved to be a safe procedure resulting in a good functional outcome. We observed a relatively high incidence of positive surgical margins. This could be attributed to a large number of extracapsular disease cases. The data regarding the prognostic factors for positive surgical margins identified in our study are the same as noted previously in the series describing LRP with BNP. Further prospective research is warranted to further investigate the effect of the size of the prostate, and the presence of a large middle lobe on the functional outcomes following laparoscopic radical prostatectomy with bladder neck sparing.