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Literature DB >> 25275778

Live fibroblast harvest reveals surface marker shift in vitro.

Graham G Walmsley¹, Yuval Rinkevich, Michael S Hu, Daniel T Montoro, David D Lo, Adrian McArdle, Zeshaan N Maan, Shane D Morrison, Dominik Duscher, Alexander J Whittam, Victor W Wong, Irving L Weissman, Geoffrey C Gurtner, Michael T Longaker.

Abstract

Current methods for the isolation of fibroblasts require extended ex vivo manipulation in cell culture. As a consequence, prior studies investigating fibroblast biology may fail to adequately represent cellular phenotypes in vivo. To overcome this problem, we describe a detailed protocol for the isolation of fibroblasts from the dorsal dermis of adult mice that bypasses the need for cell culture, thereby preserving the physiological, transcriptional, and proteomic profiles of each cell. Using the described protocol we characterized the transcriptional programs and the surface expression of 176 CD markers in cultured versus uncultured fibroblasts. The differential expression patterns we observed highlight the importance of a live harvest for investigations of fibroblast biology.

Entities: Chemical Species

Mesh：

Substances：
Biomarkers

Year: 2014 PMID： 25275778 PMCID： PMC4346232 DOI： 10.1089/ten.TEC.2014.0118

Source DB: PubMed Journal: Tissue Eng Part C Methods ISSN： 1937-3384 Impact factor: 3.056

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