| Literature DB >> 2525926 |
R D LaReau1, W Wan, V E Anderson.
Abstract
The isotope effect on binding [4-2H]NAD+ and [4-3H]NAD+ to lactate dehydrogenase has been shown to be 1.10 +/- 0.03 by whole molecule isotope ratio mass spectrometry and 1.085 +/- 0.01 by 3H/14C scintillation counting. These values demonstrate that specific interactions of the nicotinamide ring with the enzyme make the C-H bond at C-4 less stiff in the binary complex.Entities:
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Year: 1989 PMID： 2525926 DOI： 10.1021/bi00434a070
Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162