Literature DB >> 22551424

Ground-state electronic destabilization via hyperconjugation in aspartate aminotransferase.

Wait R Griswold1, Joan Nieto Castro, Andrew J Fisher, Michael D Toney.   

Abstract

Binding isotope effects for l-aspartate reacting with the inactive K258A mutant of PLP-dependent aspartate aminotransferase to give a stable external aldimine intermediate are reported. They provide direct evidence for electronic ground-state destabilization via hyperconjugation. The smaller equilibrium isotope effect with deazaPLP-reconstituted K258A indicates that the pyridine nitrogen plays an important role in labilizing the Cα-H bond.

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Year:  2012        PMID： 22551424      PMCID： PMC3425665          DOI： 10.1021/ja302809e

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  20 in total

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