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Literature DB >> 25253792

Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.

Andrea F Dugas¹, Alexandra Valsamakis², Charlotte A Gaydos³, Michael Forman², Justin Hardick⁴, Pranav Kidambi⁵, Sharmeen Amin⁵, Alisha Gupta⁵, Richard E Rothman³.

Abstract

We prospectively evaluated the performance of Cepheid's GeneXpert Xpert Flu assay in a target population of 281 adults presenting to the emergency department with an acute respiratory illness who met Centers for Disease Control and Prevention (CDC) criteria for recommended antiviral treatment. Compared with the Prodesse ProFlu+ assay, Xpert Flu had an overall sensitivity of 95.3% and specificity of 99.2%.

Entities: Disease Species

Mesh：

Year: 2014 PMID： 25253792 PMCID： PMC4313313 DOI： 10.1128/JCM.02343-14

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

1. Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.

Authors: S M Novak-Weekley; E M Marlowe; M Poulter; D Dwyer; D Speers; W Rawlinson; C Baleriola; C C Robinson
Journal: J Clin Microbiol Date: 2012-02-29 Impact factor: 5.948

2. Validation of the Cepheid Xpert Flu A real time RT-PCR detection panel for emergency use authorization.

Authors: Anthony R Sambol; Peter C Iwen; Maura Pieretti; Samik Basu; Michael H Levi; Kimberly D Gilonske; Kimberly D Moses; Jamie L Marola; Preveen Ramamoorthy
Journal: J Clin Virol Date: 2010-06-26 Impact factor: 3.168

3. Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France.

Authors: N Salez; L Ninove; L Thirion; C Gazin; C Zandotti; X de Lamballerie; R N Charrel
Journal: Clin Microbiol Infect Date: 2012-02-23 Impact factor: 8.067

Review 4. Antivirals for treatment of influenza: a systematic review and meta-analysis of observational studies.

Authors: Jonathan Hsu; Nancy Santesso; Reem Mustafa; Jan Brozek; Yao Long Chen; Jessica P Hopkins; Adrienne Cheung; Gayane Hovhannisyan; Liudmila Ivanova; Signe A Flottorp; Ingvil Saeterdal; Arthur D Wong; Jinhui Tian; Timothy M Uyeki; Elie A Akl; Pablo Alonso-Coello; Fiona Smaill; Holger J Schünemann
Journal: Ann Intern Med Date: 2012-02-27 Impact factor: 25.391

5. Performance characteristics of clinical diagnosis, a clinical decision rule, and a rapid influenza test in the detection of influenza infection in a community sample of adults.

Authors: John Stein; Janice Louie; Scott Flanders; Judith Maselli; Jill K Hacker; W Lawrence Drew; Ralph Gonzales
Journal: Ann Emerg Med Date: 2005-08-15 Impact factor: 5.721

6. Retrospective and prospective verification of the Cepheid Xpert influenza virus assay.

Authors: Elena B Popowitch; Elaine Rogers; Melissa B Miller
Journal: J Clin Microbiol Date: 2011-07-20 Impact factor: 5.948

7. Comparison of Xpert Flu rapid nucleic acid testing with rapid antigen testing for the diagnosis of influenza A and B.

Authors: Michael A DiMaio; Malaya K Sahoo; Jesse Waggoner; Benjamin A Pinsky
Journal: J Virol Methods Date: 2012-07-25 Impact factor: 2.014

8. Antiviral therapy and outcomes of influenza requiring hospitalization in Ontario, Canada.

Authors: Allison McGeer; Karen A Green; Agron Plevneshi; Altynay Shigayeva; Nilofar Siddiqi; Janet Raboud; Donald E Low
Journal: Clin Infect Dis Date: 2007-12-15 Impact factor: 9.079

9. Evaluation of the Xpert Flu A Panel nucleic acid amplification-based point-of-care test for influenza A virus detection and pandemic H1 subtyping.

Authors: Shireen L Jenny; Yaobi Hu; Pieter Overduin; Adam Meijer
Journal: J Clin Virol Date: 2010-07-31 Impact factor: 3.168

Review 10. Impact of neuraminidase inhibitor treatment on outcomes of public health importance during the 2009-2010 influenza A(H1N1) pandemic: a systematic review and meta-analysis in hospitalized patients.

Authors: Stella G Muthuri; Puja R Myles; Sudhir Venkatesan; Jo Leonardi-Bee; Jonathan S Nguyen-Van-Tam
Journal: J Infect Dis Date: 2012-11-29 Impact factor: 5.226

11 in total

1. Prospective comparison of RT-PCR/ESI-MS to Prodesse ProFlu Plus and Cepheid GenXpert for the detection of Influenza A and B viruses.

Authors: Justin Hardick; Andrea Dugas; Joshua Goheen; Richard Rothman; Charlotte Gaydos
Journal: J Virol Methods Date: 2015-02-11 Impact factor: 2.014

Review 2. Laboratory Diagnosis of Respiratory Tract Infections in Children - the State of the Art.

Authors: Shubhagata Das; Sherry Dunbar; Yi-Wei Tang
Journal: Front Microbiol Date: 2018-10-18 Impact factor: 5.640

3. Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.

Authors: Maxime Maignan; Damien Viglino; Maud Hablot; Nicolas Termoz Masson; Anne Lebeugle; Roselyne Collomb Muret; Prudence Mabiala Makele; Valérie Guglielmetti; Patrice Morand; Julien Lupo; Virginie Forget; Caroline Landelle; Sylvie Larrat
Journal: PLoS One Date: 2019-05-07 Impact factor: 3.240

Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.

1. Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.

2. Validation of the Cepheid Xpert Flu A real time RT-PCR detection panel for emergency use authorization.

3. Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France.

Review 4. Antivirals for treatment of influenza: a systematic review and meta-analysis of observational studies.

5. Performance characteristics of clinical diagnosis, a clinical decision rule, and a rapid influenza test in the detection of influenza infection in a community sample of adults.

6. Retrospective and prospective verification of the Cepheid Xpert influenza virus assay.

7. Comparison of Xpert Flu rapid nucleic acid testing with rapid antigen testing for the diagnosis of influenza A and B.

8. Antiviral therapy and outcomes of influenza requiring hospitalization in Ontario, Canada.

9. Evaluation of the Xpert Flu A Panel nucleic acid amplification-based point-of-care test for influenza A virus detection and pandemic H1 subtyping.

Review 10. Impact of neuraminidase inhibitor treatment on outcomes of public health importance during the 2009-2010 influenza A(H1N1) pandemic: a systematic review and meta-analysis in hospitalized patients.

1. Prospective comparison of RT-PCR/ESI-MS to Prodesse ProFlu Plus and Cepheid GenXpert for the detection of Influenza A and B viruses.

Review 2. Laboratory Diagnosis of Respiratory Tract Infections in Children - the State of the Art.

3. Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.

Review 4. Sample-to-result molecular infectious disease assays: clinical implications, limitations and potential.

Review 5. [Rapid diagnostic test for respiratory infections].

6. Analytical performance of the Alere™ i Influenza A&B assay for the rapid detection of influenza viruses.

7. Rapid diagnostic methods for acute viral respiratory infections.

Review 8. Diagnosing Viral and Atypical Pathogens in the Setting of Community-Acquired Pneumonia.

9. Necessity to critically review the automatic results of the Xpert Flu assay.

Review 10. Point-of-care testing for respiratory viruses in adults: The current landscape and future potential.