BACKGROUND: The implementation of diversion pouches is to minimise the risk of bacterial contamination as the initial blood flow is prevented from entering primary bag collections as it is diverted into a pouch. This study was carried out to determine the prevalence of bacterial contamination in the diversion pouches used during blood collections in the Transfusion Department of Hospital Seberang Jaya, Penang, Malaysia. METHODS: BD Bactec™ Fx instrument detection system was performed on 702 samples of 20 mL of diverting blood in diversion pouch. The inocullum volume was 10 mL for both aerobic and anaerobic bottles cultures and incubated for 5 days in the BD Bactec™ Fx instrument. Positive sample was flagged by BD Bactec™ Fx instrument and subculture to identify the species of organism. RESULTS: The results showed that of 702 samples, 12 (1.7%) were contaminated. The bacterial species identified were coagulase negative Staphylococcus, Staphylococcus aureus and Gram positive Bacilli. CONCLUSION: The results strongly suggest that the usage of diversion pouch is of significant importance in reducing bacterial contamination during blood collection.
BACKGROUND: The implementation of diversion pouches is to minimise the risk of bacterial contamination as the initial blood flow is prevented from entering primary bag collections as it is diverted into a pouch. This study was carried out to determine the prevalence of bacterial contamination in the diversion pouches used during blood collections in the Transfusion Department of Hospital Seberang Jaya, Penang, Malaysia. METHODS: BD Bactec™ Fx instrument detection system was performed on 702 samples of 20 mL of diverting blood in diversion pouch. The inocullum volume was 10 mL for both aerobic and anaerobic bottles cultures and incubated for 5 days in the BD Bactec™ Fx instrument. Positive sample was flagged by BD Bactec™ Fx instrument and subculture to identify the species of organism. RESULTS: The results showed that of 702 samples, 12 (1.7%) were contaminated. The bacterial species identified were coagulase negative Staphylococcus, Staphylococcus aureus and Gram positive Bacilli. CONCLUSION: The results strongly suggest that the usage of diversion pouch is of significant importance in reducing bacterial contamination during blood collection.
Authors: Ineke G H Rood; Dirk de Korte; Sandra Ramírez-Arcos; Paul H M Savelkoul; Annika Pettersson Journal: J Med Microbiol Date: 2011-01-06 Impact factor: 2.472
Authors: C Bruneau; P Perez; M Chassaigne; P Allouch; A Audurier; C Gulian; G Janus; G Boulard; P De Micco; L R Salmi; L Noel Journal: Transfusion Date: 2001-01 Impact factor: 3.157
Authors: Dorothy Stainsby; Hilary Jones; Deborah Asher; Claire Atterbury; Aysha Boncinelli; Lisa Brant; Catherine E Chapman; Katy Davison; Rebecca Gerrard; Alexandra Gray; Susan Knowles; Elizabeth M Love; Clare Milkins; D Brian L McClelland; Derek R Norfolk; Kate Soldan; Clare Taylor; John Revill; Lorna M Williamson; Hannah Cohen Journal: Transfus Med Rev Date: 2006-10