| Literature DB >> 25239191 |
Asima Shah1, Mudasir Ahmad1, Bilal Ahmad Ashwar1, Adil Gani2, Farooq Ahmad Masoodi1, Idrees Ahmed Wani1, Sajad Mohd Wani1, Asir Gani3.
Abstract
This paper reports the characterization and potential antioxidant activity of β-D-glucan isolated from barley treated with γ-rays. The β-D-glucan was irradiated with 0, 2, 4 and 8 kGy by gamma ray. The samples were characterized by Fourier transform-infrared spectroscopy, gel permeation chromatography (GPC) and quantitative estimation by Megazyme β-D-glucan assay kit. The average molecular weight of non-irradiated β-D-glucan was 177 kDa that decreased to 79 kDa at 8 kGy. Antioxidant activity was evaluated by five complementary assays including DPPH, lipid peroxidation, reducing power, metal chelating ability and oxidative DNA damage assays. Further, the antiproliferative potential of irradiated β-D-glucan was tested against three human cancer cell lines including Colo-205, T47D and MCF7 using MTT assay. Irradiated β-D-glucan exhibited dose dependent cancer cell growth inhibition. In conclusion, the present study demonstrates that irradiation leads to the formation of low molecular weight β-D-glucan with enhanced antioxidant and antiproliferative activities.Entities:
Keywords: Nutraceutical potential; β-d-Glucan; γ- Irradiation
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Year: 2014 PMID： 25239191 DOI： 10.1016/j.ijbiomac.2014.08.056
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953