| Literature DB >> 25214632 |
Stephen K Gire1, Augustine Goba2, Kristian G Andersen3, Rachel S G Sealfon4, Daniel J Park5, Lansana Kanneh6, Simbirie Jalloh6, Mambu Momoh7, Mohamed Fullah7, Gytis Dudas8, Shirlee Wohl9, Lina M Moses10, Nathan L Yozwiak1, Sarah Winnicki1, Christian B Matranga5, Christine M Malboeuf5, James Qu5, Adrianne D Gladden5, Stephen F Schaffner1, Xiao Yang5, Pan-Pan Jiang1, Mahan Nekoui1, Andres Colubri11, Moinya Ruth Coomber6, Mbalu Fonnie6, Alex Moigboi6, Michael Gbakie6, Fatima K Kamara6, Veronica Tucker6, Edwin Konuwa6, Sidiki Saffa6, Josephine Sellu6, Abdul Azziz Jalloh6, Alice Kovoma6, James Koninga6, Ibrahim Mustapha6, Kandeh Kargbo6, Momoh Foday6, Mohamed Yillah6, Franklyn Kanneh6, Willie Robert6, James L B Massally6, Sinéad B Chapman5, James Bochicchio5, Cheryl Murphy5, Chad Nusbaum5, Sarah Young5, Bruce W Birren5, Donald S Grant6, John S Scheiffelin10, Eric S Lander12, Christian Happi13, Sahr M Gevao14, Andreas Gnirke5, Andrew Rambaut15, Robert F Garry10, S Humarr Khan6, Pardis C Sabeti3.
Abstract
In its largest outbreak, Ebola virus disease is spreading through Guinea, Liberia, Sierra Leone, and Nigeria. We sequenced 99 Ebola virus genomes from 78 patients in Sierra Leone to ~2000× coverage. We observed a rapid accumulation of interhost and intrahost genetic variation, allowing us to characterize patterns of viral transmission over the initial weeks of the epidemic. This West African variant likely diverged from central African lineages around 2004, crossed from Guinea to Sierra Leone in May 2014, and has exhibited sustained human-to-human transmission subsequently, with no evidence of additional zoonotic sources. Because many of the mutations alter protein sequences and other biologically meaningful targets, they should be monitored for impact on diagnostics, vaccines, and therapies critical to outbreak response.Entities:
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Year: 2014 PMID： 25214632 PMCID： PMC4431643 DOI： 10.1126/science.1259657
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728