Literature DB >> 25210063

Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae.

Roger Dumke1, Enno Jacobs2.   

Abstract

Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Mesh：

Year:  2014        PMID： 25210063      PMCID： PMC4313229          DOI： 10.1128/JCM.02048-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames.

Authors:  T Dandekar; M Huynen; J T Regula; B Ueberle; C U Zimmermann; M A Andrade; T Doerks; L Sánchez-Pulido; B Snel; M Suyama; Y P Yuan; R Herrmann; P Bork
Journal:  Nucleic Acids Res       Date:  2000-09-01       Impact factor: 16.971

2.  Prevalence of Mycoplasma pneumoniae in subjectively healthy individuals.

Authors:  J Gnarpe; A Lundbäck; B Sundelöf; H Gnarpe
Journal:  Scand J Infect Dis       Date:  1992

3.  Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

Authors:  Roger Dumke; Nicol Schurwanz; Matthias Lenz; Markus Schuppler; Christian Lück; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2007-05-30       Impact factor: 5.948

4.  Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae.

Authors:  Roger Dumke; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

5.  Evaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.

Authors:  A Touati; A Benard; A Ben Hassen; C M Bébéar; S Pereyre
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

6.  Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.

Authors:  Jonas M Winchell; Kathleen A Thurman; Stephanie L Mitchell; W Lanier Thacker; Barry S Fields
Journal:  J Clin Microbiol       Date:  2008-07-09       Impact factor: 5.948

Review 7.  Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections.

Authors:  Thomas Prescott Atkinson; Mitchell F Balish; Ken B Waites
Journal:  FEMS Microbiol Rev       Date:  2008-08-27       Impact factor: 16.408

8.  Mycoplasma pneumoniae pneumonia revisited within the German Competence Network for Community-acquired pneumonia (CAPNETZ).

Authors:  Heike von Baum; Tobias Welte; Reinhard Marre; Norbert Suttorp; Christian Lück; Santiago Ewig
Journal:  BMC Infect Dis       Date:  2009-05-13       Impact factor: 3.090

9.  Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection.

Authors:  Anna C Nilsson; Per Björkman; Kenneth Persson
Journal:  BMC Microbiol       Date:  2008-06-11       Impact factor: 3.605

10.  Clinical significance of different bacterial load of Mycoplasma pneumoniae in patients with Mycoplasma pneumoniae pneumonia.

Authors:  Wujun Jiang; Yongdong Yan; Wei Ji; Yuqing Wang; Zhengrong Chen
Journal:  Braz J Infect Dis       Date:  2013-10-26       Impact factor: 3.257
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  11 in total

Review 1.  Mycoplasma pneumoniae from the Respiratory Tract and Beyond.

Authors:  Ken B Waites; Li Xiao; Yang Liu; Mitchell F Balish; T Prescott Atkinson
Journal:  Clin Microbiol Rev       Date:  2017-07       Impact factor: 26.132

2.  Evaluation of the ELITe InGenius PCR Platform for Detection of Mycoplasma pneumoniae.

Authors:  Arthur H Totten; Sixto M Leal; Amy E Ratliff; Li Xiao; Donna M Crabb; Ken B Waites
Journal:  J Clin Microbiol       Date:  2019-05-24       Impact factor: 5.948

3.  Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae.

Authors:  Sixto M Leal; Arthur H Totten; Li Xiao; Donna M Crabb; Amy Ratliff; Lynn B Duffy; Karen B Fowler; Emily Mixon; Jonas M Winchell; Maureen H Diaz; Alvaro J Benitez; Bernard J Wolff; Xuan Qin; Y-W Tang; Mark Gonzalez; Raj Selvarangan; Tao Hong; Edward Brooks; Steve Dallas; T Prescott Atkinson; Xiaotian Zheng; Jennifer Dien Bard; Ken B Waites
Journal:  J Clin Microbiol       Date:  2020-05-26       Impact factor: 5.948

4.  Rapid diagnosis of Mycoplasma pneumoniae in children with pneumonia by an immuno-chromatographic antigen assay.

Authors:  Wei Li; Yujie Liu; Yun Zhao; Ran Tao; Yonggang Li; Shiqiang Shang
Journal:  Sci Rep       Date:  2015-10-21       Impact factor: 4.379

Review 5.  The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae.

Authors:  Maureen H Diaz; Jonas M Winchell
Journal:  Front Microbiol       Date:  2016-03-08       Impact factor: 5.640

6.  Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia.

Authors:  Wei Li; You-Hong Fang; Hong-Qiang Shen; De-Hua Yang; Qiang Shu; Shi-Qiang Shang
Journal:  PLoS One       Date:  2017-05-16       Impact factor: 3.240

Review 7.  Mycoplasma pneumoniae: Current Knowledge on Nucleic Acid Amplification Techniques and Serological Diagnostics.

Authors:  Katherine Loens; Margareta Ieven
Journal:  Front Microbiol       Date:  2016-03-31       Impact factor: 5.640

8.  Th1/Th2 Cytokine Profile and Its Diagnostic Value in Mycoplasma pneumoniae Pneumonia.

Authors:  Wei Li; Yu-Jie Liu; Xiao-le Zhao; Shi-Qiang Shang; Lang Wu; Qing Ye; Hui Xu
Journal:  Iran J Pediatr       Date:  2016-01-30       Impact factor: 0.364

9.  Development of a multiplex real-time PCR assay for detection of Mycoplasma pneumoniae, Chlamydia pneumoniae and mutations associated with macrolide resistance in Mycoplasma pneumoniae from respiratory clinical specimens.

Authors:  Maaret Nummi; Laura Mannonen; Mirja Puolakkainen
Journal:  Springerplus       Date:  2015-11-10

10.  Evaluation of the illumigene Mycoplasma Direct DNA Amplification Assay.

Authors:  Neena Kanwar; Morgan A Pence; Donna Mayne; Jeffrey Michael; Rangaraj Selvarangan
Journal:  J Clin Microbiol       Date:  2018-06-25       Impact factor: 5.948
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