Literature DB >> 17537933

Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

Roger Dumke1, Nicol Schurwanz, Matthias Lenz, Markus Schuppler, Christian Lück, Enno Jacobs.   

Abstract

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

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Year:  2007        PMID： 17537933      PMCID： PMC1951254          DOI： 10.1128/JCM.00321-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Mycoplasma pneumoniae P1 type 1- and type 2-specific sequences within the P1 cytadhesin gene of individual strains.

Authors:  J W Dorigo-Zetsma; B Wilbrink; J Dankert; S A Zaat
Journal:  Infect Immun       Date:  2001-09       Impact factor: 3.441

2.  Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions.

Authors:  Martine Welti; Katia Jaton; Martin Altwegg; Roland Sahli; Aline Wenger; Jacques Bille
Journal:  Diagn Microbiol Infect Dis       Date:  2003-02       Impact factor: 2.803

Review 3.  Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections.

Authors:  K Loens; D Ursi; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

4.  Subtyping of Mycoplasma pneumoniae isolates based on extended genome sequencing and on expression profiles.

Authors:  Roger Dumke; Ina Catrein; Elsbeth Pirkil; Richard Herrmann; Enno Jacobs
Journal:  Int J Med Microbiol       Date:  2003-02       Impact factor: 3.473

5.  Detection of Mycoplasma pneumoniae in respiratory samples by real-time PCR using an inhibition control.

Authors:  Dominique Ursi; Kristien Dirven; Katherine Loens; Margareta Ieven; Herman Goossens
Journal:  J Microbiol Methods       Date:  2003-10       Impact factor: 2.363

6.  Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples.

Authors:  Roger Dumke; Paul Christian Lück; Christoph Noppen; Christoph Schaefer; Heike von Baum; Reinhard Marre; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2006-07       Impact factor: 5.948

Review 7.  Laboratory diagnosis of Mycoplasma pneumoniae infection.

Authors:  F Daxboeck; R Krause; C Wenisch
Journal:  Clin Microbiol Infect       Date:  2003-04       Impact factor: 8.067

Review 8.  Mycoplasma pneumoniae infections.

Authors:  M R Hammerschlag
Journal:  Curr Opin Infect Dis       Date:  2001-04       Impact factor: 4.915

Review 9.  Mycoplasma pneumoniae and its role as a human pathogen.

Authors:  Ken B Waites; Deborah F Talkington
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

10.  Mycoplasma pneumoniae and Legionella pneumophila in community-acquired lower respiratory tract infections among hospitalized children: diagnosis by real time PCR.

Authors:  Helen C Maltezou; Bernard La-Scola; Helen Astra; Ioanna Constantopoulou; Vasiliki Vlahou; Dimitris A Kafetzis; Andreas G Constantopoulos; Didier Raoult
Journal:  Scand J Infect Dis       Date:  2004
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  35 in total

1.  Antibiotic sensitivity of 40 Mycoplasma pneumoniae isolates and molecular analysis of macrolide-resistant isolates from Beijing, China.

Authors:  Fei Zhao; Min Lv; Xiaoxia Tao; Hui Huang; Binghua Zhang; Zhen Zhang; Jianzhong Zhang
Journal:  Antimicrob Agents Chemother       Date:  2011-11-21       Impact factor: 5.191

2.  Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae.

Authors:  Roger Dumke; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

3.  Evaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.

Authors:  A Touati; A Benard; A Ben Hassen; C M Bébéar; S Pereyre
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

4.  Sequence analysis of the p1 adhesin gene of Mycoplasma pneumoniae in clinical isolates collected in Beijing in 2008 to 2009.

Authors:  Fei Zhao; Bin Cao; Jing Li; Sufan Song; Xiaoxia Tao; Yudong Yin; Lihua He; Jianzhong Zhang
Journal:  J Clin Microbiol       Date:  2011-06-22       Impact factor: 5.948

Review 5.  Mycoplasma pneumoniae from the Respiratory Tract and Beyond.

Authors:  Ken B Waites; Li Xiao; Yang Liu; Mitchell F Balish; T Prescott Atkinson
Journal:  Clin Microbiol Rev       Date:  2017-07       Impact factor: 26.132

6.  Comparison of the illumigene Mycoplasma DNA amplification assay and culture for detection of Mycoplasma pneumoniae.

Authors:  Amy E Ratliff; Lynn B Duffy; Ken B Waites
Journal:  J Clin Microbiol       Date:  2014-01-15       Impact factor: 5.948

7.  Detection of Mycoplasma pneumoniae in simulated and true clinical throat swab specimens by nanorod array-surface-enhanced Raman spectroscopy.

Authors:  Suzanne L Hennigan; Jeremy D Driskell; Richard A Dluhy; Yiping Zhao; Ralph A Tripp; Ken B Waites; Duncan C Krause
Journal:  PLoS One       Date:  2010-10-26       Impact factor: 3.240

8.  Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens.

Authors:  Rachel R Higgins; Ernesto Lombos; Patrick Tang; Karl Rohoman; Anne Maki; Shirley Brown; Frances Jamieson; Steven J Drews
Journal:  Ann Clin Microbiol Antimicrob       Date:  2009-03-10       Impact factor: 3.944

9.  Molecular characterizations of PCR-positive Mycoplasma pneumoniae specimens collected from Australia and China.

Authors:  Guanhua Xue; Qinning Wang; Chao Yan; Neisha Jeoffreys; Liqiong Wang; Shaoli Li; Gwendolyn L Gilbert; Hongmei Sun
Journal:  J Clin Microbiol       Date:  2014-02-26       Impact factor: 5.948

10.  Mycoplasma pneumoniae pneumonia revisited within the German Competence Network for Community-acquired pneumonia (CAPNETZ).

Authors:  Heike von Baum; Tobias Welte; Reinhard Marre; Norbert Suttorp; Christian Lück; Santiago Ewig
Journal:  BMC Infect Dis       Date:  2009-05-13       Impact factor: 3.090
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