Literature DB >> 19073866

Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae.

Roger Dumke1, Enno Jacobs.   

Abstract

We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae. All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/microl, but the mean crossing points resulted in differences in the concentration of the genome copies of a factor of 20.

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Year:  2008        PMID： 19073866      PMCID： PMC2643664          DOI： 10.1128/JCM.01989-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  25 in total

1.  Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control.

Authors:  David Pitcher; Victoria J Chalker; Carmen Sheppard; Robert C George; Timothy G Harrison
Journal:  J Med Microbiol       Date:  2006-02       Impact factor: 2.472

2.  Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR.

Authors:  Karolina Gullsby; Martin Storm; Kåre Bondeson
Journal:  J Clin Microbiol       Date:  2007-12-19       Impact factor: 5.948

3.  Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

Authors:  Roger Dumke; Nicol Schurwanz; Matthias Lenz; Markus Schuppler; Christian Lück; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2007-05-30       Impact factor: 5.948

4.  Genotyping analysis of Mycoplasma pneumoniae clinical strains in Japan between 1995 and 2005: type shift phenomenon of M. pneumoniae clinical strains.

Authors:  Tsuyoshi Kenri; Norio Okazaki; Tsutomu Yamazaki; Mitsuo Narita; Kinich Izumikawa; Mayumi Matsuoka; Satowa Suzuki; Atsuko Horino; Tsuguo Sasaki
Journal:  J Med Microbiol       Date:  2008-04       Impact factor: 2.472

5.  Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.

Authors:  Jonas M Winchell; Kathleen A Thurman; Stephanie L Mitchell; W Lanier Thacker; Barry S Fields
Journal:  J Clin Microbiol       Date:  2008-07-09       Impact factor: 5.948

6.  ADP-ribosylating and vacuolating cytotoxin of Mycoplasma pneumoniae represents unique virulence determinant among bacterial pathogens.

Authors:  T R Kannan; Joel B Baseman
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-14       Impact factor: 11.205

7.  Mycoplasma pneumoniae in Korean children: the epidemiology of pneumonia over an 18-year period.

Authors:  Byung Wook Eun; Nam Hee Kim; Eun Hwa Choi; Hoan Jong Lee
Journal:  J Infect       Date:  2008-04-16       Impact factor: 6.072

8.  First report of macrolide-resistant strains and description of a novel nucleotide sequence variation in the P1 adhesin gene in Mycoplasma pneumoniae clinical strains isolated in France over 12 years.

Authors:  S Pereyre; A Charron; H Renaudin; C Bébéar; C M Bébéar
Journal:  J Clin Microbiol       Date:  2007-09-19       Impact factor: 5.948

Review 9.  Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections.

Authors:  Thomas Prescott Atkinson; Mitchell F Balish; Ken B Waites
Journal:  FEMS Microbiol Rev       Date:  2008-08-27       Impact factor: 16.408

10.  Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection.

Authors:  Anna C Nilsson; Per Björkman; Kenneth Persson
Journal:  BMC Microbiol       Date:  2008-06-11       Impact factor: 3.605
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  13 in total

1.  Evaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.

Authors:  A Touati; A Benard; A Ben Hassen; C M Bébéar; S Pereyre
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

Review 2.  Mycoplasma pneumoniae from the Respiratory Tract and Beyond.

Authors:  Ken B Waites; Li Xiao; Yang Liu; Mitchell F Balish; T Prescott Atkinson
Journal:  Clin Microbiol Rev       Date:  2017-07       Impact factor: 26.132

3.  Low rate of macrolide resistance in Mycoplasma pneumoniae strains in Germany between 2009 and 2012.

Authors:  Roger Dumke; Christian Lück; Enno Jacobs
Journal:  Antimicrob Agents Chemother       Date:  2013-05-06       Impact factor: 5.191

Review 4.  Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.

Authors:  Ken B Waites; Li Xiao; Vanya Paralanov; Rose M Viscardi; John I Glass
Journal:  J Mol Diagn       Date:  2012-07-20       Impact factor: 5.568

5.  Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae.

Authors:  Roger Dumke; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2014-09-10       Impact factor: 5.948

6.  Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.

Authors:  Elizabeth Wulff-Burchfield; Wiley A Schell; Allen E Eckhardt; Michael G Pollack; Zhishan Hua; Jeremy L Rouse; Vamsee K Pamula; Vijay Srinivasan; Jonathan L Benton; Barbara D Alexander; David A Wilfret; Monica Kraft; Charles B Cairns; John R Perfect; Thomas G Mitchell
Journal:  Diagn Microbiol Infect Dis       Date:  2010-03-12       Impact factor: 2.803

7.  Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae.

Authors:  Sixto M Leal; Arthur H Totten; Li Xiao; Donna M Crabb; Amy Ratliff; Lynn B Duffy; Karen B Fowler; Emily Mixon; Jonas M Winchell; Maureen H Diaz; Alvaro J Benitez; Bernard J Wolff; Xuan Qin; Y-W Tang; Mark Gonzalez; Raj Selvarangan; Tao Hong; Edward Brooks; Steve Dallas; T Prescott Atkinson; Xiaotian Zheng; Jennifer Dien Bard; Ken B Waites
Journal:  J Clin Microbiol       Date:  2020-05-26       Impact factor: 5.948

8.  Is Mycoplasma pneumoniae adherence to erythrocytes a factor in extrapulmonary dissemination?

Authors:  Harold Neimark; Matthew Gesner
Journal:  PLoS Pathog       Date:  2010-12-23       Impact factor: 6.823

9.  Mycoplasma pneumoniae and Chlamydia spp. infection in community-acquired pneumonia, Germany, 2011-2012.

Authors:  Roger Dumke; Christiane Schnee; Mathias W Pletz; Jan Rupp; Enno Jacobs; Konrad Sachse; Gernot Rohde
Journal:  Emerg Infect Dis       Date:  2015-03       Impact factor: 6.883

10.  Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia.

Authors:  Jiuxin Qu; Li Gu; Jiang Wu; Jianping Dong; Zenghui Pu; Yan Gao; Ming Hu; Yongxiang Zhang; Feng Gao; Bin Cao; Chen Wang
Journal:  BMC Infect Dis       Date:  2013-04-11       Impact factor: 3.090
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