Syeda Sohaila Naz1, Muhammad Raza Shah2, Nazar Ul Islam3,4, Ajmal Khan5, Samina Nazir6, Sara Qaisar7, Syed Sartaj Alam8. 1. Nanoscience and Catalysis Division, National Centre for Physics, Quaid-i-Azam University Campus, Islamabad, 44000, Pakistan. syedasohailanaz@yahoo.com. 2. H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan. raza.shah@iccs.edu. 3. Institute of Chemical Sciences, University of Peshawar, Peshawar, 25120, Pakistan. islanaz@yahoo.com. 4. Sarhad University of Science and Information Technology, Peshawar, 2500, Pakistan. islanaz@yahoo.com. 5. H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan. ajmalchemist@yahoo.com. 6. Nanoscience and Catalysis Division, National Centre for Physics, Quaid-i-Azam University Campus, Islamabad, 44000, Pakistan. seegasami01@yahoo.com. 7. Nanoscience and Catalysis Division, National Centre for Physics, Quaid-i-Azam University Campus, Islamabad, 44000, Pakistan. sara.qaisar@ncp.edu.pk. 8. Department of Plant Pathology, Khyber Pakhtunkhwa Agricultural University Peshawar, Peshawar, Pakistan. ur_sartaj@yahoo.com.
Abstract
BACKGROUND: Conjugated and drug loaded silver nanoparticles are getting an increased attention for various biomedical applications. Nanoconjugates showed significant enhancement in biological activity in comparison to free drug molecules. In this perspective, we report the synthesis of bioactive silver capped with 5-Amino-β-resorcylic acid hydrochloride dihydrate (AR). The in vitro antimicrobial (antibacterial, antifungal), enzyme inhibition (xanthine oxidase, urease, carbonic anhydrase, α-chymotrypsin, cholinesterase) and antioxidant activities of the developed nanostructures was investigated before and after conjugation to silver metal. RESULTS: The conjugation of AR to silver was confirmed through FTIR, UV-vis and TEM techniques. The amount of AR conjugated with silver was characterized through UV-vis spectroscopy and found to be 9% by weight. The stability of synthesized nanoconjugates against temperature, high salt concentration and pH was found to be good. Nanoconjugates, showed significant synergic enzyme inhibition effect against xanthine and urease enzymes in comparison to standard drugs, pure ligand and silver. CONCLUSIONS: Our synthesized nanoconjugate was found be to efficient selective xanthine and urease inhibitors in comparison to Ag and AR. On a per weight basis, our nanoconjugates required less amount of AR (about 11 times) for inhibition of these enzymes.
BACKGROUND: Conjugated and drug loaded silver nanoparticles are getting an increased attention for various biomedical applications. Nanoconjugates showed significant enhancement in biological activity in comparison to free drug molecules. In this perspective, we report the synthesis of bioactive silver capped with 5-Amino-β-resorcylic acid hydrochloride dihydrate (AR). The in vitro antimicrobial (antibacterial, antifungal), enzyme inhibition (xanthine oxidase, urease, carbonic anhydrase, α-chymotrypsin, cholinesterase) and antioxidant activities of the developed nanostructures was investigated before and after conjugation to silver metal. RESULTS: The conjugation of AR to silver was confirmed through FTIR, UV-vis and TEM techniques. The amount of AR conjugated with silver was characterized through UV-vis spectroscopy and found to be 9% by weight. The stability of synthesized nanoconjugates against temperature, high salt concentration and pH was found to be good. Nanoconjugates, showed significant synergic enzyme inhibition effect against xanthine and urease enzymes in comparison to standard drugs, pure ligand and silver. CONCLUSIONS: Our synthesized nanoconjugate was found be to efficient selective xanthine and urease inhibitors in comparison to Ag and AR. On a per weight basis, our nanoconjugates required less amount of AR (about 11 times) for inhibition of these enzymes.
Authors: S K Lee; Z H Mbwambo; H Chung; L Luyengi; E J Gamez; R G Mehta; A D Kinghorn; J M Pezzuto Journal: Comb Chem High Throughput Screen Date: 1998-04 Impact factor: 1.339
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