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Literature DB >> 25195517

Imaging lipid metabolism in live Caenorhabditis elegans using fingerprint vibrations.

Ping Wang¹, Bin Liu, Delong Zhang, Micah Y Belew, Heidi A Tissenbaum, Ji-Xin Cheng.

Abstract

Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans.

Entities: Chemical Disease Gene Species

Keywords: C. elegans; cell metabolism; lipids; stimulated Raman scattering; vibrational spectroscopy

Mesh：

Substances：
Lipids

Year: 2014 PMID： 25195517 PMCID： PMC4348091 DOI： 10.1002/anie.201406029

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

26 in total

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Journal: Proc Natl Acad Sci U S A Date: 2007-09-05 Impact factor: 11.205

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Journal: Nat Genet Date: 2006-02-05 Impact factor: 38.330

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Authors: P Greenspan; S D Fowler
Journal: J Lipid Res Date: 1985-07 Impact factor: 5.922

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Authors: Kaveh Ashrafi; Francesca Y Chang; Jennifer L Watts; Andrew G Fraser; Ravi S Kamath; Julie Ahringer; Gary Ruvkun
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10. Fast vibrational imaging of single cells and tissues by stimulated Raman scattering microscopy.

Authors: Delong Zhang; Ping Wang; Mikhail N Slipchenko; Ji-Xin Cheng
Journal: Acc Chem Res Date: 2014-05-28 Impact factor: 22.384

20 in total

1. Rapid spontaneous Raman light sheet microscopy using cw-lasers and tunable filters.

Authors: Israel Rocha-Mendoza; Jacob Licea-Rodriguez; Mónica Marro; Omar E Olarte; Marcos Plata-Sanchez; Pablo Loza-Alvarez
Journal: Biomed Opt Express Date: 2015-08-19 Impact factor: 3.732

2. Label-Free Imaging of Heme Dynamics in Living Organisms by Transient Absorption Microscopy.

Authors: Andy Jing Chen; Xiaojing Yuan; Junjie Li; Puting Dong; Iqbal Hamza; Ji-Xin Cheng
Journal: Anal Chem Date: 2018-02-14 Impact factor: 6.986

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Authors: Chi Zhang; Delong Zhang; Ji-Xin Cheng
Journal: Annu Rev Biomed Eng Date: 2015-10-22 Impact factor: 9.590

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Authors: Ming Sheng; András Gorzsás; Simon Tuck
Journal: Worm Date: 2016-02-18

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Authors: Chien-Sheng Liao; Ji-Xin Cheng
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Review 6. Mammalian cell and tissue imaging using Raman and coherent Raman microscopy.

Authors: Anthony A Fung; Lingyan Shi
Journal: Wiley Interdiscip Rev Syst Biol Med Date: 2020-07-19

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Authors: Shuhua Yue; Ji-Xin Cheng
Journal: Curr Opin Chem Biol Date: 2016-06-08 Impact factor: 8.822

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Authors: Yinxin Zhang; Chien-Sheng Liao; Weili Hong; Kai-Chih Huang; Huaidong Yang; Guofan Jin; Ji-Xin Cheng
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Authors: Min Hi Park; Jeffrey L Suhalim; Firas Elmastour; Santu K Singha; Tadashi Imafuku; Ramanathan Venkatnarayan; Anette Christ; Alena Grebe; Sarah A Oppelt; Dmitri Sviridov; Michael Bukrinsky; Eicke Latz; Eric O Potma; Michael L Fitzgerald
Journal: PLoS One Date: 2021-05-13 Impact factor: 3.240

10. Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning.

Authors: Haonan Lin; Hyeon Jeong Lee; Nathan Tague; Jean-Baptiste Lugagne; Cheng Zong; Fengyuan Deng; Jonghyeon Shin; Lei Tian; Wilson Wong; Mary J Dunlop; Ji-Xin Cheng
Journal: Nat Commun Date: 2021-05-24 Impact factor: 14.919