Yu Hua Wong1, Wai Yan Tan1, Chin Ping Tan2, Kamariah Long3, Kar Lin Nyam1. 1. Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia. 2. Department of Food Technology, Faculty of Food Science and Technology,Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 3. Malaysian Agricultural Research & Development Institute (MARDI) PO Box 12301, 50774 Kuala Lumpur, Malaysia.
Abstract
OBJECTIVE: To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines. METHODS: The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope. RESULTS: The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected. CONCLUSIONS: KSE and KSO could be potential sources of natural anti-cancer agents. Further investigations on using kenaf seeds for anti-proliferative properties are warranted.
OBJECTIVE: To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, humanbreast cancer, humancolon cancer and humanlung cancer cell lines. METHODS: The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on humancancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope. RESULTS: The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected. CONCLUSIONS:KSE and KSO could be potential sources of natural anti-cancer agents. Further investigations on using kenaf seeds for anti-proliferative properties are warranted.
Entities:
Keywords:
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; HeLa; Human breast cancer; Human cervical cancer; Human colon cancer; Human lung cancer; Sulforhodamine B assay
Authors: Márcia Carvalho; Pedro J Ferreira; Vanda S Mendes; Renata Silva; José A Pereira; Carmen Jerónimo; Branca M Silva Journal: Food Chem Toxicol Date: 2009-10-31 Impact factor: 6.023