SCOPE: We have previously shown an increase in adipocyte size and lipid content in retroperitoneal white adipose tissue (rWAT) induced by an 8-week high-sugar diet (HSD). In this study, we assessed the effect of a HSD on the transcriptional activity of adipogenic genes in a time-course study to provide insight regarding the genetic networks involved in the rWAT response to dietary sugar. METHODS AND RESULTS: Weaned male Wistar rats were fed a standard chow diet or HSD (68% carbohydrates) for 4, 8 or 12 weeks, and rWAT was removed for histopathology and PCR array (adipogenesis) analyses. The HSD induced adipocyte hypertrophy and hyperplasia in rWAT after 12 weeks of ingestion. Additionally, the HSD altered serum VLDL-cholesterol, triacylglycerol and glucometabolic parameters. Hierarchical clustering revealed HSD-induced changes in the expression patterns of the tested gene set. Pathway analysis, which used the enrichment analysis algorithm of the Thompson Reuters MetaCore platform, associated a cluster of differentially expressed genes with canonical pathways related to regulating adipocyte differentiation and proliferation (p-value < 10(-7)). CONCLUSION: HSD feeding post-weaning increased both the adipocyte size and number by simultaneously up-regulating pro-adipogenic signals (the PPARγ pathway) and down-regulating anti-adipogenic signals (Wnt pathway) in young adults.
SCOPE: We have previously shown an increase in adipocyte size and lipid content in retroperitoneal white adipose tissue (rWAT) induced by an 8-week high-sugar diet (HSD). In this study, we assessed the effect of a HSD on the transcriptional activity of adipogenic genes in a time-course study to provide insight regarding the genetic networks involved in the rWAT response to dietary sugar. METHODS AND RESULTS: Weaned male Wistar rats were fed a standard chow diet or HSD (68% carbohydrates) for 4, 8 or 12 weeks, and rWAT was removed for histopathology and PCR array (adipogenesis) analyses. The HSD induced adipocyte hypertrophy and hyperplasia in rWAT after 12 weeks of ingestion. Additionally, the HSD altered serum VLDL-cholesterol, triacylglycerol and glucometabolic parameters. Hierarchical clustering revealed HSD-induced changes in the expression patterns of the tested gene set. Pathway analysis, which used the enrichment analysis algorithm of the Thompson Reuters MetaCore platform, associated a cluster of differentially expressed genes with canonical pathways related to regulating adipocyte differentiation and proliferation (p-value < 10(-7)). CONCLUSION:HSD feeding post-weaning increased both the adipocyte size and number by simultaneously up-regulating pro-adipogenic signals (the PPARγ pathway) and down-regulating anti-adipogenic signals (Wnt pathway) in young adults.
Authors: Maria Andrea Barbosa; Renata Guerra-Sá; Uberdan Guilherme Mendes De Castro; Wanderson Geraldo de Lima; Robson Augusto Souza Dos Santos; Maria José Campagnole-Santos; Andréia Carvalho Alzamora Journal: J Physiol Biochem Date: 2018-05-25 Impact factor: 4.158
Authors: Rosângela Maria Lopes Sousa; Nathalee Liberal Xavier Ribeiro; Bruno Araújo Serra Pinto; Jonas Rodrigues Sanches; Mariana Uchôa da Silva; Caio Fernando Ferreira Coêlho; Lucas Martins França; José Albuquerque de Figueiredo Neto; Antonio Marcus de Andrade Paes Journal: Nutr Metab (Lond) Date: 2018-07-24 Impact factor: 4.169