Literature DB >> 25143580

Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species.

Barbara A Qurollo1, Dana Riggins1, Alaire Comyn1, Merone T Zewde1, Edward B Breitschwerdt2.   

Abstract

We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 25143580      PMCID： PMC4313244          DOI： 10.1128/JCM.02340-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Infection with Panola Mountain Ehrlichia sp. in a dog with atypical lymphocytes and clonal T-cell expansion.

Authors:  B A Qurollo; A C Davenport; B M Sherbert; C B Grindem; A J Birkenheuer; E B Breitschwerdt
Journal:  J Vet Intern Med       Date:  2013-07-22       Impact factor: 3.333

2.  Comparison of PCR assays for detection of the agent of human granulocytic ehrlichiosis, Anaplasma phagocytophilum.

Authors:  Robert F Massung; Kimetha G Slater
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

3.  Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, and Ehrlichia species in dogs from Minnesota.

Authors:  Melissa J Beall; Ramaswamy Chandrashekar; Matthew D Eberts; Katie E Cyr; Pedro Paulo V P Diniz; Celine Mainville; Barbara C Hegarty; John M Crawford; Edward B Breitschwerdt
Journal:  Vector Borne Zoonotic Dis       Date:  2008-08       Impact factor: 2.133

4.  Evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for Ehrlichia, Anaplasma, spotted fever group Rickettsia, Bartonella, and Borrelia species in canine neurological diseases (109 cases).

Authors:  R M Barber; Q Li; P P V P Diniz; B F Porter; E B Breitschwerdt; M K Claiborne; A J Birkenheuer; J M Levine; G J Levine; K Chandler; P Kenny; P Nghiem; S Wei; C E Greene; M Kent; S R Platt; K Greer; S J Schatzberg
Journal:  J Vet Intern Med       Date:  2010 Mar-Apr       Impact factor: 3.333

5.  Development of loop-mediated isothermal amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium.

Authors:  Ryo Nakao; Ellen Y Stromdahl; Joseph W Magona; Bonto Faburay; Boniface Namangala; Imna Malele; Noboru Inoue; Dirk Geysen; Kiichi Kajino; Frans Jongejan; Chihiro Sugimoto
Journal:  BMC Microbiol       Date:  2010-11-19       Impact factor: 3.605
  5 in total
  8 in total

1.  Attenuating the Selection of Vancomycin Resistance Among Enterococci through the Development of Peptide-Based Vancomycin Antagonists.

Authors:  Ryan W Mull; Alec A Brennan; Brittany R Russ; Yftah Tal-Gan
Journal:  ACS Infect Dis       Date:  2020-10-01       Impact factor: 5.084

2.  Bartonella rochalimae Detection by a Sensitive and Specific PCR Platform.

Authors:  Dennis Chan; Joseph Andrew Geiger; Elton José Rosas Vasconcelos; Brian Oakley; Pedro Paulo Vissotto de Paiva Diniz
Journal:  Am J Trop Med Hyg       Date:  2018-10       Impact factor: 2.345

3.  Serological and molecular analysis of feline vector-borne anaplasmosis and ehrlichiosis using species-specific peptides and PCR.

Authors:  Barbara C Hegarty; Barbara A Qurollo; Brittany Thomas; Karen Park; Ramaswamy Chandrashekar; Melissa J Beall; Brendon Thatcher; Edward B Breitschwerdt
Journal:  Parasit Vectors       Date:  2015-06-12       Impact factor: 3.876

4.  Naturally Occurring Ehrlichia ewingii and Mycoplasma sp. Co-Infection in a Goat.

Authors:  K Meichner; B A Qurollo; K L Anderson; C B Grindem; M Savage; E B Breitschwerdt
Journal:  J Vet Intern Med       Date:  2015-10-16       Impact factor: 3.333

5.  Prevalence of Vector-Borne Pathogens in Southern California Dogs With Clinical and Laboratory Abnormalities Consistent With Immune-Mediated Disease.

Authors:  L Kidd; B Qurollo; M Lappin; K Richter; J R Hart; S Hill; C Osmond; E B Breitschwerdt
Journal:  J Vet Intern Med       Date:  2017-05-30       Impact factor: 3.333

6.  Evidence for vertical transmission of Mycoplasma haemocanis, but not Ehrlichia ewingii, in a dog.

Authors:  Erin Lashnits; Sandra Grant; Brittany Thomas; Barbara Qurollo; Edward B Breitschwerdt
Journal:  J Vet Intern Med       Date:  2019-05-24       Impact factor: 3.333

7.  Molecular Evidence and Hematological Profile of Bovines Naturally Infected with Ehrlichiosis in Southern Punjab, Pakistan.

Authors:  Muhammad Abdul Basit; Muhammad Ijaz; Jawaria Ali Khan; Kamran Ashraf; Rao Zahid Abbas
Journal:  Acta Parasitol       Date:  2021-06-17       Impact factor: 1.440

8.  A simple and rapid procedure for the detection of genes encoding Shiga toxins and other specific DNA sequences.

Authors:  Bożena Nejman-Faleńczyk; Sylwia Bloch; Aleksandra Januszkiewicz; Alicja Węgrzyn; Grzegorz Węgrzyn
Journal:  Toxins (Basel)       Date:  2015-11-13       Impact factor: 4.546
  8 in total

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