BACKGROUND: Vector-transmitted microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, Bartonella, and Borrelia are commonly suspected in dogs with meningoencephalomyelitis (MEM), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (CSF) of dogs with MEM is unknown. HYPOTHESIS/ OBJECTIVES: To determine if DNA from these genera is present in brain tissue and CSF of dogs with MEM, including those with meningoencephalitis of unknown etiology (MUE) and histopathologically confirmed cases of granulomatous (GME) and necrotizing meningoencephalomyelitis (NME). ANIMALS: Hundred and nine dogs examined for neurological signs at 3 university referral hospitals. METHODS: Brain tissue and CSF were collected prospectively from dogs with neurological disease and evaluated by broadly reactive polymerase chain reaction (PCR) for Ehrlichia, Anaplasma, Spotted Fever Group Rickettsia, Bartonella, and Borrelia species. Medical records were evaluated retrospectively to identify MEM and control cases. RESULTS: Seventy-five cases of MUE, GME, or NME, including brain tissue from 31 and CSF from 44 cases, were evaluated. Brain tissue from 4 cases and inflammatory CSF from 30 cases with infectious, neoplastic, compressive, vascular, or malformative disease were evaluated as controls. Pathogen nucleic acids were detected in 1 of 109 cases evaluated. Specifically, Bartonella vinsonii subsp. berkhoffii DNA was amplified from 1/6 dogs with histopathologically confirmed GME. CONCLUSION AND CLINICAL IMPORTANCE: The results of this investigation suggest that microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, and Borrelia are unlikely to be directly associated with canine MEM in the geographic regions evaluated. The role of Bartonella in the pathogenesis of GME warrants further investigation.
BACKGROUND: Vector-transmitted microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, Bartonella, and Borrelia are commonly suspected in dogs with meningoencephalomyelitis (MEM), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (CSF) of dogs with MEM is unknown. HYPOTHESIS/ OBJECTIVES: To determine if DNA from these genera is present in brain tissue and CSF of dogs with MEM, including those with meningoencephalitis of unknown etiology (MUE) and histopathologically confirmed cases of granulomatous (GME) and necrotizing meningoencephalomyelitis (NME). ANIMALS: Hundred and nine dogs examined for neurological signs at 3 university referral hospitals. METHODS: Brain tissue and CSF were collected prospectively from dogs with neurological disease and evaluated by broadly reactive polymerase chain reaction (PCR) for Ehrlichia, Anaplasma, Spotted Fever Group Rickettsia, Bartonella, and Borrelia species. Medical records were evaluated retrospectively to identify MEM and control cases. RESULTS: Seventy-five cases of MUE, GME, or NME, including brain tissue from 31 and CSF from 44 cases, were evaluated. Brain tissue from 4 cases and inflammatory CSF from 30 cases with infectious, neoplastic, compressive, vascular, or malformative disease were evaluated as controls. Pathogen nucleic acids were detected in 1 of 109 cases evaluated. Specifically, Bartonella vinsonii subsp. berkhoffii DNA was amplified from 1/6 dogs with histopathologically confirmed GME. CONCLUSION AND CLINICAL IMPORTANCE: The results of this investigation suggest that microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, and Borrelia are unlikely to be directly associated with canine MEM in the geographic regions evaluated. The role of Bartonella in the pathogenesis of GME warrants further investigation.
Authors: Barbara A Qurollo; Dana Riggins; Alaire Comyn; Merone T Zewde; Edward B Breitschwerdt Journal: J Clin Microbiol Date: 2014-08-20 Impact factor: 5.948
Authors: Joseph Geiger; Bridget A Morton; Elton Jose Rosas Vasconcelos; Maryam Tngrian; Malika Kachani; Eduardo A Barrón; Cesar M Gavidia; Robert H Gilman; Noelia P Angulo; Richard Lerner; Tamerin Scott; N Hannah Mirrashed; Brian Oakley; Pedro Paulo V P Diniz Journal: Am J Trop Med Hyg Date: 2018-06-21 Impact factor: 2.345
Authors: M Lanza-Perea; U Zieger; B A Qurollo; B C Hegarty; E L Pultorak; S Kumthekar; R Bruhl-Day; E B Breitschwerdt Journal: J Vet Intern Med Date: 2014-10-01 Impact factor: 3.333
Authors: Domenico Otranto; Gabriella Testini; Filipe Dantas-Torres; Maria S Latrofa; Pedro Paulo Vissotto de Paiva Diniz; Donato de Caprariis; Riccardo P Lia; Norbert Mencke; Dorothee Stanneck; Gioia Capelli; Edward B Breitschwerdt Journal: J Clin Microbiol Date: 2010-07-21 Impact factor: 5.948
Authors: Pedro Paulo V P Diniz; Bridget A Morton; Maryam Tngrian; Malika Kachani; Eduardo A Barrón; Cesar M Gavidia; Robert H Gilman; Noelia P Angulo; Elliott C Brenner; Richard Lerner; Bruno B Chomel Journal: PLoS Negl Trop Dis Date: 2013-09-05
Authors: Isabelle Schrauwen; Renee M Barber; Scott J Schatzberg; Ashley L Siniard; Jason J Corneveaux; Brian F Porter; Karen M Vernau; Rebekah I Keesler; Kaspar Matiasek; Thomas Flegel; Andrew D Miller; Teresa Southard; Christopher L Mariani; Gayle C Johnson; Matthew J Huentelman Journal: PLoS One Date: 2014-11-13 Impact factor: 3.240
Authors: Filipe Dantas-Torres; Gioia Capelli; Alessio Giannelli; Rafael Antonio Nascimento Ramos; Riccardo Paolo Lia; Cinzia Cantacessi; Donato de Caprariis; Anna Sara De Tommasi; Maria Stefania Latrofa; Vita Lacasella; Viviana Domenica Tarallo; Giancarlo Di Paola; Barbara Qurollo; Edward Breitschwerdt; Dorothee Stanneck; Domenico Otranto Journal: Parasit Vectors Date: 2013-08-23 Impact factor: 3.876
Authors: Francesco Dondi; Samanta Russo; Chiara Agnoli; Nicola Mengoli; Andrea Balboni; Alberto Alberti; Mara Battilani Journal: ScientificWorldJournal Date: 2014-06-05