Muhammad Abdul Basit1, Muhammad Ijaz2, Jawaria Ali Khan1, Kamran Ashraf3, Rao Zahid Abbas4. 1. Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan. 2. Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan. mijaz@uvas.edu.pk. 3. Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan. 4. Department of Parasitology, University of Agriculture, Faisalabad, 38040, Pakistan.
Abstract
PURPOSE: Ehrlichiosis is one of the tick-borne diseases, which is not only neglected in Pakistan but only a few reports have been documented throughout the globe. The purpose of this study is to highlight and report the neglected pathogen from bovines in Pakistan. METHODS: In this study, the pathogen was detected initially based on microscopy, followed by the molecular confirmation and phylogenetic analysis of the pathogen from bovines from south Punjab, Pakistan. The hematological parameters were also assessed in Ehrlichia positive and negative animals. The information of different disease determinants was analyzed by a logistic regression model on SPSS. RESULTS: The study has reported an 11.98% (23/192) prevalence of Ehrlichiosis from bovines. The prevalence was slightly more in cattle (13.5%) as compared to the buffaloes (10.4%). The previous tick's history and tick control methods were proved to be the key factors with the occurrence of the disease. The isolates from Pakistan Ehrlichia spp. Pakistan/31, 36, and 8 clustered with the isolates from the USA, Nicaraguan, France, South Africa, and Uganda. Platelet count, hemoglobin level, and hematocrit were found significantly decreased in Ehrlichia affected animals when compared to the healthy bovines. CONCLUSION: This is the first report of ehrlichiosis from bovines in Pakistan and will provide the roadmap for future research.
PURPOSE: Ehrlichiosis is one of the tick-borne diseases, which is not only neglected in Pakistan but only a few reports have been documented throughout the globe. The purpose of this study is to highlight and report the neglected pathogen from bovines in Pakistan. METHODS: In this study, the pathogen was detected initially based on microscopy, followed by the molecular confirmation and phylogenetic analysis of the pathogen from bovines from south Punjab, Pakistan. The hematological parameters were also assessed in Ehrlichia positive and negative animals. The information of different disease determinants was analyzed by a logistic regression model on SPSS. RESULTS: The study has reported an 11.98% (23/192) prevalence of Ehrlichiosis from bovines. The prevalence was slightly more in cattle (13.5%) as compared to the buffaloes (10.4%). The previous tick's history and tick control methods were proved to be the key factors with the occurrence of the disease. The isolates from Pakistan Ehrlichia spp. Pakistan/31, 36, and 8 clustered with the isolates from the USA, Nicaraguan, France, South Africa, and Uganda. Platelet count, hemoglobin level, and hematocrit were found significantly decreased in Ehrlichia affected animals when compared to the healthy bovines. CONCLUSION: This is the first report of ehrlichiosis from bovines in Pakistan and will provide the roadmap for future research.
Authors: Barbara A Qurollo; Dana Riggins; Alaire Comyn; Merone T Zewde; Edward B Breitschwerdt Journal: J Clin Microbiol Date: 2014-08-20 Impact factor: 5.948
Authors: Awais Ghaffar; Muhammad Ijaz; Ahmad Ali; Shahid Hussain Farooqi; Abdul Rehman; Muhammad Muddassir Ali; Muhammad Zeeshan Zafar; Muhammad Anas Naeem Journal: J Parasitol Date: 2020-06-01 Impact factor: 1.276