| Literature DB >> 25126991 |
Takayuki Miki1, Sho-hei Fujishima1, Kazuhiro Komatsu1, Keiko Kuwata2, Shigeki Kiyonaka1, Itaru Hamachi3.
Abstract
The functions of membrane proteins are tightly controlled by the dynamics such as protein trafficking and degradation. We demonstrated that ligand-directed acyl imidazole (LDAI) chemistry is broadly applicable to selective chemical labeling of various types of membrane-bound proteins under live cell conditions without a need for any tag fragments. The LDAI chemistry enabled pulse-chase analysis of these proteins to determine the half-life, as well as their degradation pathways by the imaging, under almost natural cellular conditions.Entities:
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Year: 2014 PMID： 25126991 DOI： 10.1016/j.chembiol.2014.07.013
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521