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Literature DB >> 8327482

An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.

V Azevedo¹, E Alvarez, E Zumstein, G Damiani, V Sgaramella, S D Ehrlich, P Serror.

Abstract

A collection of 772 Bacillus subtilis DNA segments was obtained by cloning in yeast artificial chromosomes. The B. subtilis inserts of 288 clones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in four contigs that cover > 98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.

Entities: Species

Mesh：

Substances：
DNA, Bacterial

Year: 1993 PMID： 8327482 PMCID： PMC46864 DOI： 10.1073/pnas.90.13.6047

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

32 in total

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