Literature DB >> 25086504

Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes.

Signe Mathiasen1, Sune M Christensen1, Juan José Fung2, Søren G F Rasmussen3, Jonathan F Fay4, Sune K Jorgensen1, Salome Veshaguri1, David L Farrens4, Maria Kiskowski5, Brian Kobilka6, Dimitrios Stamou1.   

Abstract

Proteoliposome reconstitution is a standard method to stabilize purified transmembrane proteins in membranes for structural and functional assays. Here we quantified intrareconstitution heterogeneities in single proteoliposomes using fluorescence microscopy. Our results suggest that compositional heterogeneities can severely skew ensemble-average proteoliposome measurements but also enable ultraminiaturized high-content screens. We took advantage of this screening capability to map the oligomerization energy of the β2-adrenergic receptor using ∼10(9)-fold less protein than conventional assays.

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Year:  2014        PMID： 25086504      PMCID： PMC4485457          DOI： 10.1038/nmeth.3062

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  36 in total

1.  Optimizing methods to recover absolute FRET efficiency from immobilized single molecules.

Authors:  James J McCann; Ucheor B Choi; Liqiang Zheng; Keith Weninger; Mark E Bowen
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Review 2.  How many drug targets are there?

Authors:  John P Overington; Bissan Al-Lazikani; Andrew L Hopkins
Journal:  Nat Rev Drug Discov       Date:  2006-12       Impact factor: 84.694

Review 3.  Single vesicle biochips for ultra-miniaturized nanoscale fluidics and single molecule bioscience.

Authors:  Andreas L Christensen; Christina Lohr; Sune M Christensen; Dimitrios Stamou
Journal:  Lab Chip       Date:  2013-09-21       Impact factor: 6.799

4.  New fluorescent dyes in the red region for biodiagnostics.

Authors:  M Sauer; K T Han; R Müller; S Nord; A Schulz; S Seeger; J Wolfrum; J Arden-Jacob; G Deltau; N J Marx; C Zander; K H Drexhage
Journal:  J Fluoresc       Date:  1995-09       Impact factor: 2.217

Review 5.  Artificial membrane-like environments for in vitro studies of purified G-protein coupled receptors.

Authors:  Eugene Serebryany; Gefei Alex Zhu; Elsa C Y Yan
Journal:  Biochim Biophys Acta       Date:  2011-08-09

6.  A key agonist-induced conformational change in the cannabinoid receptor CB1 is blocked by the allosteric ligand Org 27569.

Authors:  Jonathan F Fay; David L Farrens
Journal:  J Biol Chem       Date:  2012-07-30       Impact factor: 5.157

Review 7.  The prevalence, maintenance, and relevance of G protein-coupled receptor oligomerization.

Authors:  Graeme Milligan
Journal:  Mol Pharmacol       Date:  2013-04-30       Impact factor: 4.436

8.  Topology and quaternary structure of pro-sucrase/isomaltase and final-form sucrase/isomaltase.

Authors:  G M Cowell; J Tranum-Jensen; H Sjöström; O Norén
Journal:  Biochem J       Date:  1986-07-15       Impact factor: 3.857

9.  Cysteine residues in the human cannabinoid receptor: only C257 and C264 are required for a functional receptor, and steric bulk at C386 impairs antagonist SR141716A binding.

Authors:  Jonathan F Fay; Thomas D Dunham; David L Farrens
Journal:  Biochemistry       Date:  2005-06-21       Impact factor: 3.162

10.  Ligand-regulated oligomerization of beta(2)-adrenoceptors in a model lipid bilayer.

Authors:  Juan José Fung; Xavier Deupi; Leonardo Pardo; Xiao Jie Yao; Gisselle A Velez-Ruiz; Brian T Devree; Roger K Sunahara; Brian K Kobilka
Journal:  EMBO J       Date:  2009-09-17       Impact factor: 11.598
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  25 in total

1.  Conformational dynamics between transmembrane domains and allosteric modulation of a metabotropic glutamate receptor.

Authors:  Vanessa A Gutzeit; Jordana Thibado; Daniel Starer Stor; Zhou Zhou; Scott C Blanchard; Olaf S Andersen; Joshua Levitz
Journal:  Elife       Date:  2019-06-07       Impact factor: 8.140

2.  Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells.

Authors:  Kadla R Rosholm; Natascha Leijnse; Anna Mantsiou; Vadym Tkach; Søren L Pedersen; Volker F Wirth; Lene B Oddershede; Knud J Jensen; Karen L Martinez; Nikos S Hatzakis; Poul Martin Bendix; Andrew Callan-Jones; Dimitrios Stamou
Journal:  Nat Chem Biol       Date:  2017-05-08       Impact factor: 15.040

3.  Understanding the FRET Signatures of Interacting Membrane Proteins.

Authors:  Christopher King; Valerica Raicu; Kalina Hristova
Journal:  J Biol Chem       Date:  2017-02-09       Impact factor: 5.157

4.  Single Proteoliposome High-Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs.

Authors:  Samuel M Walsh; Signe Mathiasen; Sune M Christensen; Jonathan F Fay; Christopher King; Davide Provasi; Ernesto Borrero; Søren G F Rasmussen; Juan Jose Fung; Marta Filizola; Kalina Hristova; Brian Kobilka; David L Farrens; Dimitrios Stamou
Journal:  Biophys J       Date:  2018-07-17       Impact factor: 4.033

5.  Dynamics of P-type ATPase transport revealed by single-molecule FRET.

Authors:  Mateusz Dyla; Daniel S Terry; Magnus Kjaergaard; Thomas L-M Sørensen; Jacob Lauwring Andersen; Jens P Andersen; Charlotte Rohde Knudsen; Roger B Altman; Poul Nissen; Scott C Blanchard
Journal:  Nature       Date:  2017-11-08       Impact factor: 49.962

6.  The dimerization equilibrium of a ClC Cl(-)/H(+) antiporter in lipid bilayers.

Authors:  Rahul Chadda; Venkatramanan Krishnamani; Kacey Mersch; Jason Wong; Marley Brimberry; Ankita Chadda; Ludmila Kolmakova-Partensky; Larry J Friedman; Jeff Gelles; Janice L Robertson
Journal:  Elife       Date:  2016-08-03       Impact factor: 8.140

7.  Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy.

Authors:  R Chadda; J L Robertson
Journal:  Methods Enzymol       Date:  2016-10-11       Impact factor: 1.600

8.  Direct observation of proton pumping by a eukaryotic P-type ATPase.

Authors:  Salome Veshaguri; Sune M Christensen; Gerdi C Kemmer; Garima Ghale; Mads P Møller; Christina Lohr; Andreas L Christensen; Bo H Justesen; Ida L Jørgensen; Jürgen Schiller; Nikos S Hatzakis; Michael Grabe; Thomas Günther Pomorski; Dimitrios Stamou
Journal:  Science       Date:  2016-03-25       Impact factor: 47.728

9.  Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR.

Authors:  He Tian; Thomas P Sakmar; Thomas Huber
Journal:  Chembiochem       Date:  2015-05-11       Impact factor: 3.164

Review 10.  Biophysical aspects of biomineralization.

Authors:  Maytê Bolean; Ana M S Simão; Marina B Barioni; Bruno Z Favarin; Heitor G Sebinelli; Ekeveliny A Veschi; Tatiane A B Janku; Massimo Bottini; Marc F Hoylaerts; Rosangela Itri; José L Millán; Pietro Ciancaglini
Journal:  Biophys Rev       Date:  2017-08-29
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