| Literature DB >> 28481347 |
Kadla R Rosholm1,2, Natascha Leijnse2,3, Anna Mantsiou1,2, Vadym Tkach1,2, Søren L Pedersen2,4, Volker F Wirth1,2, Lene B Oddershede2,3, Knud J Jensen2,4, Karen L Martinez1,2, Nikos S Hatzakis1,2, Poul Martin Bendix3, Andrew Callan-Jones5, Dimitrios Stamou1,2.
Abstract
The targeted spatial organization (sorting) of Gprotein-coupled receptors (GPCRs) is essential for their biological function and often takes place in highly curved membrane compartments such as filopodia, endocytic pits, trafficking vesicles or endosome tubules. However, the influence of geometrical membrane curvature on GPCR sorting remains unknown. Here we used fluorescence imaging to establish a quantitative correlation between membrane curvature and sorting of three prototypic class A GPCRs (the neuropeptide Y receptor Y2, the β1 adrenergic receptor and the β2 adrenergic receptor) in living cells. Fitting of a thermodynamic model to the data enabled us to quantify how sorting is mediated by an energetic drive to match receptor shape and membrane curvature. Curvature-dependent sorting was regulated by ligands in a specific manner. We anticipate that this curvature-dependent biomechanical coupling mechanism contributes to the sorting, trafficking and function of transmembrane proteins in general.Entities:
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Year: 2017 PMID： 28481347 DOI： 10.1038/nchembio.2372
Source DB: PubMed Journal: Nat Chem Biol ISSN： 1552-4450 Impact factor: 15.040