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Literature DB >> 20851971

Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.

Abstract

We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.

Entities: Chemical Species

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Year: 2010 PMID： 20851971 PMCID： PMC2976206 DOI： 10.1128/AEM.01456-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

28 in total

1. Gene modifications and mutation mapping to study the function of photosystem II.

Authors: W F Vermaas
Journal: Methods Enzymol Date: 1998 Impact factor: 1.600

2. Transformation of a filamentous cyanobacterium by electroporation.

Authors: T Thiel; H Poo
Journal: J Bacteriol Date: 1989-10 Impact factor: 3.490

3. Nuclear-gene targeting by using single-stranded DNA avoids illegitimate DNA integration in Chlamydomonas reinhardtii.

Authors: Boris Zorin; Peter Hegemann; Irina Sizova
Journal: Eukaryot Cell Date: 2005-07

4. Conjugal transfer of DNA to cyanobacteria.

Authors: J Elhai; C P Wolk
Journal: Methods Enzymol Date: 1988 Impact factor: 1.600

5. A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase.

Authors: F B Simpson; R H Burris
Journal: Science Date: 1984-06-08 Impact factor: 47.728

6. Transcriptional and translational regulation of photosystem I and II genes in light-dark- and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142.

Authors: M S Colón-López; L A Sherman
Journal: J Bacteriol Date: 1998-02 Impact factor: 3.490

7. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers.

Authors: J Elhai; C P Wolk
Journal: Gene Date: 1988-08-15 Impact factor: 3.688

8. Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142.

Authors: M A Schneegurt; D M Sherman; S Nayar; L A Sherman
Journal: J Bacteriol Date: 1994-03 Impact factor: 3.490

9. Differential transcriptional analysis of the cyanobacterium Cyanothece sp. strain ATCC 51142 during light-dark and continuous-light growth.

Authors: Jörg Toepel; Eric Welsh; Tina C Summerfield; Himadri B Pakrasi; Louis A Sherman
Journal: J Bacteriol Date: 2008-04-04 Impact factor: 3.490

12 in total

1. Transcriptional regulators ChlR and CnfR are essential for diazotrophic growth in nonheterocystous cyanobacteria.

Authors: Ryoma Tsujimoto; Narumi Kamiya; Yuichi Fujita
Journal: Proc Natl Acad Sci U S A Date: 2014-04-21 Impact factor: 11.205

2. Diurnal rhythm of a unicellular diazotrophic cyanobacterium under mixotrophic conditions and elevated carbon dioxide.

Authors: Sandeep B Gaudana; Swathi Alagesan; Madhu Chetty; Pramod P Wangikar
Journal: Photosynth Res Date: 2013-07-25 Impact factor: 3.573

3. Metabolic pathways for photobiological hydrogen production by nitrogenase- and hydrogenase-containing unicellular cyanobacteria Cyanothece.

Authors: Nicholas J Skizim; Gennady M Ananyev; Anagha Krishnan; G Charles Dismukes
Journal: J Biol Chem Date: 2011-11-29 Impact factor: 5.157

4. The uptake hydrogenase in the unicellular diazotrophic cyanobacterium Cyanothece sp. strain PCC 7822 protects nitrogenase from oxygen toxicity.

Authors: Xiaohui Zhang; Debra M Sherman; Louis A Sherman
Journal: J Bacteriol Date: 2013-12-06 Impact factor: 3.490

5. Enhanced Nitrogen Fixation in a glgX-Deficient Strain of Cyanothece sp. Strain ATCC 51142, a Unicellular Nitrogen-Fixing Cyanobacterium.

Authors: Michelle Liberton; Anindita Bandyopadhyay; Himadri B Pakrasi
Journal: Appl Environ Microbiol Date: 2019-03-22 Impact factor: 4.792

6. Proteome analyses of strains ATCC 51142 and PCC 7822 of the diazotrophic cyanobacterium Cyanothece sp. under culture conditions resulting in enhanced H₂ production.

Authors: Uma K Aryal; Stephen J Callister; Sujata Mishra; Xiaohui Zhang; Janani I Shutthanandan; Thomas E Angel; Anil K Shukla; Matthew E Monroe; Ronald J Moore; David W Koppenaal; Richard D Smith; Louis Sherman
Journal: Appl Environ Microbiol Date: 2012-11-30 Impact factor: 4.792

7. Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822.

Authors: David G Welkie; Debra M Sherman; William B Chrisler; Galya Orr; Louis A Sherman
Journal: Photosynth Res Date: 2013-10-19 Impact factor: 3.573

8. Altering the Structure of Carbohydrate Storage Granules in the Cyanobacterium Synechocystis sp. Strain PCC 6803 through Branching-Enzyme Truncations.

Authors: David G Welkie; Byung-Hoo Lee; Louis A Sherman
Journal: J Bacteriol Date: 2015-12-14 Impact factor: 3.490

9. Genome-scale modeling of light-driven reductant partitioning and carbon fluxes in diazotrophic unicellular cyanobacterium Cyanothece sp. ATCC 51142.

Authors: Trang T Vu; Sergey M Stolyar; Grigoriy E Pinchuk; Eric A Hill; Leo A Kucek; Roslyn N Brown; Mary S Lipton; Andrei Osterman; Jim K Fredrickson; Allan E Konopka; Alexander S Beliaev; Jennifer L Reed
Journal: PLoS Comput Biol Date: 2012-04-05 Impact factor: 4.475

10. Rapid construction of metabolic models for a family of Cyanobacteria using a multiple source annotation workflow.

Authors: Thomas J Mueller; Bertram M Berla; Himadri B Pakrasi; Costas D Maranas
Journal: BMC Syst Biol Date: 2013-12-27