Literature DB >> 25056837

A new manual dispensing system for in meso membrane protein crystallization with using a stepping motor-based dispenser.

Masakatsu Hato1, Toshiaki Hosaka, Hiroaki Tanabe, Tokuji Kitsunai, Shigeyuki Yokoyama.   

Abstract

A reliable and easy to use manual dispensing system has been developed for the in meso membrane protein crystallization method. The system consists of a stepping motor-based dispenser with a new microsyringe system for dispensing, which allows us to deliver any desired volume of highly viscous lipidic mesophase in the range from ~50 to at least ~200 nl. The average, standard deviation, and coefficient of variation of 20 repeated deliveries of 50 nl cubic phase were comparable to those of a current robotic dispensing. Moreover, the bottom faces of boluses delivered to the glass crystallization plate were reproducibly circular in shape, and their centers were within about 100 μm from the center of the crystallization well. The system was useful for crystallizing membrane and soluble proteins in meso.

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Year:  2014        PMID： 25056837      PMCID： PMC4125823          DOI： 10.1007/s10969-014-9187-9

Source DB:  PubMed          Journal:  J Struct Funct Genomics        ISSN： 1345-711X


  9 in total

1.  A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases.

Authors:  Vadim Cherezov; Avinash Peddi; Lalitha Muthusubramaniam; Yuan F Zheng; Martin Caffrey
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-09-23

2.  High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.

Authors:  Vadim Cherezov; Daniel M Rosenbaum; Michael A Hanson; Søren G F Rasmussen; Foon Sun Thian; Tong Sun Kobilka; Hee-Jung Choi; Peter Kuhn; William I Weis; Brian K Kobilka; Raymond C Stevens
Journal:  Science       Date:  2007-10-25       Impact factor: 47.728

Review 3.  Crystallizing membrane proteins for structure determination: use of lipidic mesophases.

Authors:  Martin Caffrey
Journal:  Annu Rev Biophys       Date:  2009       Impact factor: 12.981

4.  Lipidic cubic phases: a novel concept for the crystallization of membrane proteins.

Authors:  E M Landau; J P Rosenbusch
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-10       Impact factor: 11.205

5.  Aqueous phase behavior of a 1-O-phytanyl-beta-D-xyloside/water system. Glycolipid-based bicontinuous cubic phases of crystallographic space groups Pn3m and Ia3d.

Authors:  Masakatsu Hato; Itaru Yamashita; Tadashi Kato; Yutaka Abe
Journal:  Langmuir       Date:  2004-12-21       Impact factor: 3.882

Review 6.  Lipidic cubic phase technologies for membrane protein structural studies.

Authors:  Vadim Cherezov
Journal:  Curr Opin Struct Biol       Date:  2011-07-19       Impact factor: 6.809

7.  Aqueous phase behavior of lipids with isoprenoid type hydrophobic chains.

Authors:  Masakatsu Hato; Jun Yamashita; Manzo Shiono
Journal:  J Phys Chem B       Date:  2009-07-30       Impact factor: 2.991

8.  Crystallizing membrane proteins using lipidic mesophases.

Authors:  Martin Caffrey; Vadim Cherezov
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

9.  New lipid family that forms inverted cubic phases in equilibrium with excess water: molecular structure-aqueous phase structure relationship for lipids with 5,9,13,17-tetramethyloctadecyl and 5,9,13,17-tetramethyloctadecanoyl chains.

Authors:  Jun Yamashita; Manzo Shiono; Masakatsu Hato
Journal:  J Phys Chem B       Date:  2008-09-06       Impact factor: 2.991
  9 in total
  5 in total

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Journal:  Nat Chem Biol       Date:  2018-01-08       Impact factor: 15.040

2.  Structural Mechanism for Light-driven Transport by a New Type of Chloride Ion Pump, Nonlabens marinus Rhodopsin-3.

Authors:  Toshiaki Hosaka; Susumu Yoshizawa; Yu Nakajima; Noboru Ohsawa; Masakatsu Hato; Edward F DeLong; Kazuhiro Kogure; Shigeyuki Yokoyama; Tomomi Kimura-Someya; Wataru Iwasaki; Mikako Shirouzu
Journal:  J Biol Chem       Date:  2016-06-30       Impact factor: 5.157

3.  Crystal structures of the human adiponectin receptors.

Authors:  Miki Okada-Iwabu; Masato Iwabu; Yoshihiro Nakamura; Hiroaki Tanabe; Yoshifumi Fujii; Toshiaki Hosaka; Kanna Motoyama; Mariko Ikeda; Motoaki Wakiyama; Takaho Terada; Noboru Ohsawa; Masakatsu Hato; Satoshi Ogasawara; Tomoya Hino; Takeshi Murata; So Iwata; Kunio Hirata; Yoshiaki Kawano; Masaki Yamamoto; Tomomi Kimura-Someya; Mikako Shirouzu; Toshimasa Yamauchi; Takashi Kadowaki; Shigeyuki Yokoyama
Journal:  Nature       Date:  2015-04-08       Impact factor: 49.962

4.  Expression, purification, crystallization, and preliminary X-ray crystallographic studies of the human adiponectin receptors, AdipoR1 and AdipoR2.

Authors:  Hiroaki Tanabe; Kanna Motoyama; Mariko Ikeda; Motoaki Wakiyama; Takaho Terada; Noboru Ohsawa; Toshiaki Hosaka; Masakatsu Hato; Yoshifumi Fujii; Yoshihiro Nakamura; Satoshi Ogasawara; Tomoya Hino; Takeshi Murata; So Iwata; Miki Okada-Iwabu; Masato Iwabu; Kunio Hirata; Yoshiaki Kawano; Masaki Yamamoto; Tomomi Kimura-Someya; Mikako Shirouzu; Toshimasa Yamauchi; Takashi Kadowaki; Shigeyuki Yokoyama
Journal:  J Struct Funct Genomics       Date:  2015-01-10

5.  Isoprenoid-chained lipid EROCOC17+4: a new matrix for membrane protein crystallization and a crystal delivery medium in serial femtosecond crystallography.

Authors:  Kentaro Ihara; Masakatsu Hato; Takanori Nakane; Keitaro Yamashita; Tomomi Kimura-Someya; Toshiaki Hosaka; Yoshiko Ishizuka-Katsura; Rie Tanaka; Tomoyuki Tanaka; Michihiro Sugahara; Kunio Hirata; Masaki Yamamoto; Osamu Nureki; Kensuke Tono; Eriko Nango; So Iwata; Mikako Shirouzu
Journal:  Sci Rep       Date:  2020-11-09       Impact factor: 4.379
  5 in total

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