Pradeep Mr1, Yadavalli Guruprasad2, Maji Jose3, Kartikay Saxena4, Deepa K1, Vishnudas Prabhu5. 1. Senior Lecturer, Department of Oral Pathology & Microbiology, AME'S Dental College, Hospital and Research Institute , Raichur, Karnataka, India . 2. Reader, Department of Oral Pathology & Microbiology, AME'S Dental College, Hospital and Research Institute , Raichur, Karnataka, India . 3. Professor and Head, Department of Oral Pathology & Microbiology, Yenepoya Dental College and Hospital , Mangalore, Karnataka, India . 4. Senior Lecturer, Department of Oral Pathology & Microbiology, Yenepoya Dental College and Hospital , Mangalore, Karnataka, India . 5. Professor, Department of Oral Pathology & Microbiology, Yenepoya Dental College and Hospital , Mangalore, Karnataka, India .
Abstract
BACKGROUND: Oral cancer is one of the most debilitating diseases afflicting mankind. Consumption of tobacco in various forms constitutes one of the most important etiological factors in initiation of oral cancer. When the focus of today's research is to determine early genotoxic changes in human cells, micronucleus (MN) assay provides a simple, yet reliable indicator of genotoxic damage. AIMS AND OBJECTIVES: To identify and quantify micronuclei in the exfoliated cells of oral mucosa in individuals with different tobacco related habits and control group, to compare the genotoxicity of different tobacco related habits between each group and also with that of control group. PATIENTS AND METHODS: In the present study buccal smears of 135 individuals with different tobacco related habits & buccal smears of 45 age and sex matched controls were obtained, stained using Giemsa stain and then observed under 100X magnification in order to identify and quantify micronuclei in the exfoliated cells of oral mucosa. RESULTS: The mean Micronucleus (MN) count in individuals having smoking habit were 3.11 while the count was 0.50, 2.13, and 1.67 in normal control, smoking with beetle quid and smokeless tobacco habit respectively. MN count in smokers group was 2.6 times more compared to normal controls. MN count was more even in other groups when compared to normal control but to a lesser extent. CONCLUSION: From our study we concluded that tobacco in any form is genotoxic especially smokers are of higher risk and micronucleus assay can be used as a simple yet reliable marker for genotoxic evaluation.
BACKGROUND:Oral cancer is one of the most debilitating diseases afflicting mankind. Consumption of tobacco in various forms constitutes one of the most important etiological factors in initiation of oral cancer. When the focus of today's research is to determine early genotoxic changes in human cells, micronucleus (MN) assay provides a simple, yet reliable indicator of genotoxic damage. AIMS AND OBJECTIVES: To identify and quantify micronuclei in the exfoliated cells of oral mucosa in individuals with different tobacco related habits and control group, to compare the genotoxicity of different tobacco related habits between each group and also with that of control group. PATIENTS AND METHODS: In the present study buccal smears of 135 individuals with different tobacco related habits & buccal smears of 45 age and sex matched controls were obtained, stained using Giemsa stain and then observed under 100X magnification in order to identify and quantify micronuclei in the exfoliated cells of oral mucosa. RESULTS: The mean Micronucleus (MN) count in individuals having smoking habit were 3.11 while the count was 0.50, 2.13, and 1.67 in normal control, smoking with beetle quid and smokeless tobacco habit respectively. MN count in smokers group was 2.6 times more compared to normal controls. MN count was more even in other groups when compared to normal control but to a lesser extent. CONCLUSION: From our study we concluded that tobacco in any form is genotoxic especially smokers are of higher risk and micronucleus assay can be used as a simple yet reliable marker for genotoxic evaluation.
Authors: O Torres-Bugarín; A De Anda-Casillas; M P Ramírez-Muñoz; J Sánchez-Corona; J M Cantú; G Zúñiga Journal: Mutat Res Date: 1998-03-30 Impact factor: 2.433