Literature DB >> 24974029

Light sheet microscopy.

Michael Weber1, Michaela Mickoleit1, Jan Huisken1.   

Abstract

This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample rotation. Based on our experience over the last 10 years, we summarize the key concepts in light sheet microscopy, typical implementations, and successful applications. In particular, sample mounting for long time-lapse imaging and the resulting challenges in data processing are discussed in detail.
© 2014 Elsevier Inc. All rights reserved.

Keywords:  Developmental biology; Fluorescence microscopy; Image processing; Light sheet microscopy; Mounting; Selective plane illumination microscopy; Three-dimensional imaging; Ultramicroscopy; Zebrafish

Mesh:

Year:  2014        PMID: 24974029     DOI: 10.1016/B978-0-12-420138-5.00011-2

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN: 0091-679X            Impact factor:   1.441


  24 in total

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2.  Faster and less phototoxic 3D fluorescence microscopy using a versatile compressed sensing scheme.

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Journal:  Biophys J       Date:  2016-09-20       Impact factor: 4.033

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Authors:  Andrew G Cox; Allison Tsomides; Andrew J Kim; Diane Saunders; Katie L Hwang; Kimberley J Evason; Jerry Heidel; Kristin K Brown; Min Yuan; Evan C Lien; Byung Cheon Lee; Sahar Nissim; Bryan Dickinson; Sagar Chhangawala; Christopher J Chang; John M Asara; Yariv Houvras; Vadim N Gladyshev; Wolfram Goessling
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Authors:  Andrew G Cox; Allison Tsomides; Dean Yimlamai; Katie L Hwang; Joel Miesfeld; Giorgio G Galli; Brendan H Fowl; Michael Fort; Kimberly Y Ma; Mark R Sullivan; Aaron M Hosios; Erin Snay; Min Yuan; Kristin K Brown; Evan C Lien; Sagar Chhangawala; Matthew L Steinhauser; John M Asara; Yariv Houvras; Brian Link; Matthew G Vander Heiden; Fernando D Camargo; Wolfram Goessling
Journal:  EMBO J       Date:  2018-10-22       Impact factor: 11.598

7.  To the Cloud! A Grassroots Proposal to Accelerate Brain Science Discovery.

Authors: 
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8.  The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis.

Authors:  Alexandra B Schroeder; Ellen T A Dobson; Curtis T Rueden; Pavel Tomancak; Florian Jug; Kevin W Eliceiri
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9.  Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time.

Authors:  Natalie Andrews; Marie-Christine Ramel; Sunil Kumar; Yuriy Alexandrov; Douglas J Kelly; Sean C Warren; Louise Kerry; Nicola Lockwood; Antonina Frolov; Paul Frankel; Laurence Bugeon; James McGinty; Margaret J Dallman; Paul M W French
Journal:  J Biophotonics       Date:  2016-01-11       Impact factor: 3.207

10.  Physically-based in silico light sheet microscopy for visualizing fluorescent brain models.

Authors:  Marwan Abdellah; Ahmet Bilgili; Stefan Eilemann; Henry Markram; Felix Schürmann
Journal:  BMC Bioinformatics       Date:  2015-08-13       Impact factor: 3.169

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