| Literature DB >> 24970769 |
Ji-fei Yang1, You-quan Li1, Zhi-jie Liu1, Jun-long Liu1, Gui-quan Guan1, Ze Chen1, Jian-xun Luo1, Xiao-long Wang2, Hong Yin3.
Abstract
DNA from liver samples of 17 free-ranging wild Reeves' muntjac (Muntiacus reevesi) was used for PCR amplification of piropalsm 18S rRNA gene. Of 17 samples, 14 (82.4%) showed a specific PCR product which were cloned and sequenced. BLAST analysis of the sequences obtained showed similarities to Babesia sp., Theileria capreoli, Theileria uilenbergi and Theileria sp. BO302-SE. Phylogenetic analysis showed that the Babesia sp. detected in the present study was distantly separated from known Babesia species of wild and domestic animals. Six sequences showed 100% similarity to T. capreoli while five sequences were separated from all known Theileria species and constituted an independent clade with Theileria sp. BO302-SE derived from roe deer in Italy; two sequences were close to T. uilenbergi with 97% similarity. This is the first description of hemoparasite infection in free-ranging wild Reeves' muntjac in China. Our results indicate that wild Reeves' muntjac may play an important reservoir role for hemoparasites. CrownEntities:
Keywords: 18S rRNA gene; Babesia; Phylogeny; Reeves' muntjac; Theileria
Mesh：
Substances：
Year: 2014 PMID： 24970769 DOI： 10.1016/j.parint.2014.06.002
Source DB: PubMed Journal: Parasitol Int ISSN： 1383-5769 Impact factor: 2.230