| Literature DB >> 24933124 |
Carola Engler1, Mark Youles, Ramona Gruetzner, Tim-Martin Ehnert, Stefan Werner, Jonathan D G Jones, Nicola J Patron, Sylvestre Marillonnet.
Abstract
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.Entities:
Keywords: Agrobacterium tumefaciens; gene assembly; genetic engineering; molecular cloning; plant biotechnology; vector construction
Mesh：
Year: 2014 PMID： 24933124 DOI： 10.1021/sb4001504
Source DB: PubMed Journal: ACS Synth Biol ISSN： 2161-5063 Impact factor: 5.110