Paula Volpato Sanitá1, Chaiene Evelin Zago2, Ewerton Garcia de Oliveira Mima3, Ana Cláudia Pavarina4, Janaina Habib Jorge3, Ana Lúcia Machado5, Carlos Eduardo Vergani6. 1. Substitute Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. 2. PhD Student, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. 3. Assistant Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. 4. Associate Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. 5. Full Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. 6. Full Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil. Electronic address: vergani@foar.unesp.br.
Abstract
OBJECTIVE: To evaluate the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP) by Candida glabrata and C tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates). STUDY DESIGN: After incubation, the supernatants and pellets of the isolates were used for the enzymatic assays and quantification of colony-forming units (CFU), respectively. Colorimetric tests were used with phosphatidylcholine as a substrate for PL and azocasein as a substrate for SAP, and the absorbances of the samples were measured. Enzymatic rates were calculated, and values were normalized by CFU. Results were analyzed with factorial analyses of variance (α = .05). RESULTS: C tropicalis and C glabrata were proteolytic and phospholipolytic. The clinical sources of isolates had no significant effect on the enzymatic activities (P > .05). C tropicalis had significantly higher enzymatic activity for both PL and SAP (P < .001) than did C glabrata. CONCLUSIONS: C tropicalis isolates produced significantly higher amounts of both enzymes than did the C glabrata isolates.
OBJECTIVE: To evaluate the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP) by Candida glabrata and C tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates). STUDY DESIGN: After incubation, the supernatants and pellets of the isolates were used for the enzymatic assays and quantification of colony-forming units (CFU), respectively. Colorimetric tests were used with phosphatidylcholine as a substrate for PL and azocasein as a substrate for SAP, and the absorbances of the samples were measured. Enzymatic rates were calculated, and values were normalized by CFU. Results were analyzed with factorial analyses of variance (α = .05). RESULTS: C tropicalis and C glabrata were proteolytic and phospholipolytic. The clinical sources of isolates had no significant effect on the enzymatic activities (P > .05). C tropicalis had significantly higher enzymatic activity for both PL and SAP (P < .001) than did C glabrata. CONCLUSIONS: C tropicalis isolates produced significantly higher amounts of both enzymes than did the C glabrata isolates.
Authors: João X S Neto; Mirella L Pereira; Jose T A Oliveira; Lady C B Rocha-Bezerra; Tiago D P Lopes; Helen P S Costa; Daniele O B Sousa; Bruno A M Rocha; Thalles B Grangeiro; José E C Freire; Ana Cristina O Monteiro-Moreira; Marina D P Lobo; Raimunda S N Brilhante; Ilka M Vasconcelos Journal: Front Microbiol Date: 2017-06-06 Impact factor: 5.640
Authors: Maroun M Sfeir; Cristina Jiménez-Ortigosa; Maria N Gamaletsou; Audrey N Schuetz; Rosemary Soave; Koen Van Besien; Catherine B Small; David S Perlin; Thomas J Walsh Journal: J Fungi (Basel) Date: 2020-01-31