Paola Ayala Ramírez1, Reggie García Robles1, Juan Diego Rojas2, Martha Bermúdez1, Jaime Bernal1. 1. Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia. E-mail: rgarcia@javeriana.edu.co. 2. Department of Obstetrics and Gynecology Pontificia Universidad Javeriana-Hospital Universitario San Ignacio, Bogotá, D.C., Colombia. And Gynecologist physician. Unit of Feto-maternal MedicineSS.E-mail: jdiegorojas@yahoo.com.
Abstract
OBJECTIVE: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies. METHODS: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase. RESULTS: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically significant differences between the values of total RNA extracted from plasma (p= 0.5975) nor in the messenger RNA expression of hPL gene (p= 0.5785) between cases and controls. CONCLUSION: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.
OBJECTIVE: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies. METHODS: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (HumanPlacental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase. RESULTS: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically significant differences between the values of total RNA extracted from plasma (p= 0.5975) nor in the messenger RNA expression of hPL gene (p= 0.5785) between cases and controls. CONCLUSION: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.
Authors: Winnie W I Pang; Michelle H Y Tsui; Daljit Sahota; Tak Y Leung; Tze K Lau; Y M Dennis Lo; Rossa W K Chiu Journal: Prenat Diagn Date: 2009-05 Impact factor: 3.050
Authors: Nancy B Y Tsui; Blenda C K Wong; Tak Y Leung; Tze K Lau; Rossa W K Chiu; Y M Dennis Lo Journal: Prenat Diagn Date: 2009-11 Impact factor: 3.050
Authors: Rossa W K Chiu; Wing-bong Lui; Mei-chun Cheung; Nihal Kumta; Antonio Farina; Irina Banzola; Silvia Grotti; Nicola Rizzo; Christopher J Haines; Y M Dennis Lo Journal: Clin Chem Date: 2006-02 Impact factor: 8.327