Literature DB >> 24814248

Ratio of intracellular precursors concentration and their flux influences hyaluronic acid molecular weight in Streptococcus zooepidemicus and recombinant Lactococcus lactis.

Sneh Sanjay Badle1, Guhan Jayaraman1, K B Ramachandran2.   

Abstract

HA molecular weight variation in Streptococcus zooepidemicus and two recombinant Lactococcus lactis strains were investigated by chemostat experiments and metabolic flux analysis (MFA). The study showed that intracellular flux ratio of UDP-GlcUA to UDP-GlcNAc correlated directly with HA molecular weight, for all the three strains. The ratio of intracellular concentration of these HA precursors also exhibited a similar trend. Phosphoglucoisomerase activity and glucose flux towards lactic acid formation were found to be the major bottlenecks for HA production in all the three strains. The study suggests that environmental conditions and genetic manipulations that balance the intracellular flux and HA precursors concentrations will result in increased molecular weight.
Copyright © 2014 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Hyaluronic acid; Lactococcus lactis; MFA; Molecular weight; Streptococcus zooepidemicus

Mesh：

Substances：

Year:  2014        PMID： 24814248     DOI： 10.1016/j.biortech.2014.04.027

Source DB:  PubMed          Journal:  Bioresour Technol        ISSN： 0960-8524            Impact factor:   9.642


  8 in total

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Journal:  Mol Biotechnol       Date:  2017-06       Impact factor: 2.695

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Authors:  Mandeep Kaur; Guhan Jayaraman
Journal:  Metab Eng Commun       Date:  2016-01-22

5.  Engineering S. equi subsp. zooepidemicus towards concurrent production of hyaluronic acid and chondroitin biopolymers of biomedical interest.

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Journal:  AMB Express       Date:  2017-03-14       Impact factor: 3.298

6.  Optimization of scleroglucan production by Sclerotium rolfsii by lowering pH during fermentation via oxalate metabolic pathway manipulation using CRISPR/Cas9.

Authors:  Tianlong Bai; Teng Wang; Yan Li; Na L Gao; Lixin Zhang; Wei-Hua Chen; Xiushan Yin
Journal:  Fungal Biol Biotechnol       Date:  2021-02-18

7.  Evaluation of Ogataea (Hansenula) polymorpha for Hyaluronic Acid Production.

Authors:  João Heitor Colombelli Manfrão-Netto; Enzo Bento Queiroz; Kelly Assis Rodrigues; Cintia M Coelho; Hugo Costa Paes; Elibio Leopoldo Rech; Nádia Skorupa Parachin
Journal:  Microorganisms       Date:  2021-02-03

Review 8.  Heterologous production of chondroitin.

Authors:  Márcia R Couto; Joana L Rodrigues; Lígia R Rodrigues
Journal:  Biotechnol Rep (Amst)       Date:  2022-02-10
  8 in total

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