Literature DB >> 2479829

Identification of a new promoter upstream of the murine dihydrofolate reductase gene.

L J Schilling1, P J Farnham.   

Abstract

In vitro reactions identified a transcription initiation site located 740 nucleotides upstream of the dihydrofolate reductase translational start. Transcription from this site proceeded in the direction opposite to that of dihydrofolate reductase mRNA. Deletion mapping indicated that this new promoter can be separated from the dihydrofolate reductase promoter and that separation increased transcription at -740. Transcripts that initiate at -740 were also detected in cellular RNA, indicating that this is a bona fide transcription initiation site in vivo.

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Year:  1989        PMID： 2479829      PMCID： PMC362546          DOI： 10.1128/mcb.9.10.4568-4570.1989

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  13 in total

1.  Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.

Authors:  P J Farnham; J M Abrams; R T Schimke
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

2.  Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle.

Authors:  P J Farnham; R T Schimke
Journal:  J Biol Chem       Date:  1985-06-25       Impact factor: 5.157

3.  A re-examination of the 5' termini of mouse dihydrofolate reductase RNA.

Authors:  S Sazer; R T Schimke
Journal:  J Biol Chem       Date:  1986-04-05       Impact factor: 5.157

4.  In vitro transcription and delimitation of promoter elements of the murine dihydrofolate reductase gene.

Authors:  P J Farnham; R T Schimke
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

5.  Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

Authors:  J Favaloro; R Treisman; R Kamen
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Abundant nuclear ribonucleoprotein form of CAD RNA.

Authors:  R Sperling; J Sperling; A D Levine; P Spann; G R Stark; R D Kornberg
Journal:  Mol Cell Biol       Date:  1985-03       Impact factor: 4.272

7.  Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene.

Authors:  G F Crouse; E J Leys; R N McEwan; E G Frayne; R E Kellems
Journal:  Mol Cell Biol       Date:  1985-08       Impact factor: 4.272

8.  The functional human dihydrofolate reductase gene.

Authors:  M J Chen; T Shimada; A D Moulton; A Cline; R K Humphries; J Maizel; A W Nienhuis
Journal:  J Biol Chem       Date:  1984-03-25       Impact factor: 5.157

9.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

10.  Cell cycle regulation of transfected murine dihydrofolate reductase genes.

Authors:  C S Gasser; R T Schimke
Journal:  J Biol Chem       Date:  1986-05-25       Impact factor: 5.157
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  7 in total

1.  Evidence that USF can interact with only a single general transcription complex at one time.

Authors:  G Adami; L E Babiss
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

2.  Sp1 activation of RNA polymerase II transcription complexes involves a heat-labile DNA-binding component.

Authors:  P J Farnham; M M Cornwell
Journal:  Gene Expr       Date:  1991-05

3.  Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site.

Authors:  A L Means; P J Farnham
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

4.  Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter.

Authors:  P J Farnham; A L Means
Journal:  Mol Cell Biol       Date:  1990-04       Impact factor: 4.272

5.  Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter.

Authors:  A Gavalas; J E Dixon; K A Brayton; H Zalkin
Journal:  Mol Cell Biol       Date:  1993-08       Impact factor: 4.272

6.  The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II.

Authors:  A B Buermeyer; N E Thompson; L A Strasheim; R R Burgess; P J Farnham
Journal:  Mol Cell Biol       Date:  1992-05       Impact factor: 4.272

7.  Protein-DNA interactions at the major and minor promoters of the divergently transcribed dhfr and rep3 genes during the Chinese hamster ovary cell cycle.

Authors:  J Wells; P Held; S Illenye; N H Heintz
Journal:  Mol Cell Biol       Date:  1996-02       Impact factor: 4.272
  7 in total

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