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Literature DB >> 24797424

CRISPR transcriptional repression devices and layered circuits in mammalian cells.

Samira Kiani¹, Jacob Beal², Mohammad R Ebrahimkhani³, Jin Huh⁴, Richard N Hall¹, Zhen Xie⁵, Yinqing Li⁶, Ron Weiss⁷.

Abstract

A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：

Year: 2014 PMID： 24797424 PMCID： PMC4228775 DOI： 10.1038/nmeth.2969

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

21 in total

1. A versatile single-plasmid system for tissue-specific and inducible control of gene expression in transgenic mice.

Authors: Jae-Kyun Ko; Kyoung-Han Choi; Xiaoli Zhao; Shinji Komazaki; Zui Pan; Noah Weisleder; Jianjie Ma
Journal: FASEB J Date: 2011-04-25 Impact factor: 5.191

Review 2. Control of rRNA synthesis in Escherichia coli: a systems biology approach.

Authors: Patrick P Dennis; Mans Ehrenberg; Hans Bremer
Journal: Microbiol Mol Biol Rev Date: 2004-12 Impact factor: 11.056

Review 3. Synthetic biology moving into the clinic.

Authors: Warren C Ruder; Ting Lu; James J Collins
Journal: Science Date: 2011-09-02 Impact factor: 47.728

4. Growth-rate-dependent partitioning of RNA polymerases in bacteria.

Authors: Stefan Klumpp; Terence Hwa
Journal: Proc Natl Acad Sci U S A Date: 2008-12-10 Impact factor: 11.205

5. siRNA-mediated gene silencing in vitro and in vivo.

Authors: Haibin Xia; Qinwen Mao; Henry L Paulson; Beverly L Davidson
Journal: Nat Biotechnol Date: 2002-09-16 Impact factor: 54.908

6. Expression of shRNA from a tissue-specific pol II promoter is an effective and safe RNAi therapeutic.

Authors: Jeffery C Giering; Dirk Grimm; Theresa A Storm; Mark A Kay
Journal: Mol Ther Date: 2008-07-29 Impact factor: 11.454

7. A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution.

Authors: Shi-Lung Lin; Donald Chang; Da-Yu Wu; Shao-Yao Ying
Journal: Biochem Biophys Res Commun Date: 2003-10-24 Impact factor: 3.575

8. BioLogic gates enable logical transcription control in mammalian cells.

Authors: Beat P Kramer; Cornelius Fischer; Martin Fussenegger
Journal: Biotechnol Bioeng Date: 2004-08-20 Impact factor: 4.530

9. Synthetic biology: new engineering rules for an emerging discipline.

Authors: Ernesto Andrianantoandro; Subhayu Basu; David K Karig; Ron Weiss
Journal: Mol Syst Biol Date: 2006-05-16 Impact factor: 11.429

10. Comparison of RNAi efficiency mediated by tetracycline-responsive H1 and U6 promoter variants in mammalian cell lines.

Authors: Jørn R Henriksen; Cecilie Løkke; Martin Hammerø; Dirk Geerts; Rogier Versteeg; Trond Flaegstad; Christer Einvik
Journal: Nucleic Acids Res Date: 2007-04-10 Impact factor: 16.971

77 in total

CRISPR transcriptional repression devices and layered circuits in mammalian cells.

1. A versatile single-plasmid system for tissue-specific and inducible control of gene expression in transgenic mice.

Review 2. Control of rRNA synthesis in Escherichia coli: a systems biology approach.

Review 3. Synthetic biology moving into the clinic.

4. Growth-rate-dependent partitioning of RNA polymerases in bacteria.

5. siRNA-mediated gene silencing in vitro and in vivo.

6. Expression of shRNA from a tissue-specific pol II promoter is an effective and safe RNAi therapeutic.

7. A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution.

8. BioLogic gates enable logical transcription control in mammalian cells.

9. Synthetic biology: new engineering rules for an emerging discipline.

10. Comparison of RNAi efficiency mediated by tetracycline-responsive H1 and U6 promoter variants in mammalian cell lines.

1. Target specificity of the CRISPR-Cas9 system.

2. Creating small transcription activating RNAs.

Review 3. CRISPR-Cas type II-based Synthetic Biology applications in eukaryotic cells.

4. Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.

5. Optogenetic characterization methods overcome key challenges in synthetic and systems biology.

Review 6. Control theory meets synthetic biology.

7. CRISPR: gene editing is just the beginning.

Review 8. Precision Control of CRISPR-Cas9 Using Small Molecules and Light.

9. Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design.

Review 10. Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications.