Literature DB >> 2479289

Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells.

J Karlinsey1, G Stamatoyannopoulos, T Enver.   

Abstract

An extraction procedure for the simultaneous isolation of RNA and DNA from tissue culture cells is described. The procedure is a variation of the guanidium/lithium chloride method for RNA isolation which is rapid, simple, and avoids costly ultracentrifugation equipment. The genomic DNA yielded by this procedure is greater than 50 kb in length and may be readily cleaved by restriction endonucleases. Sufficient DNA for Southern blot analysis, and RNA for Northern blot or nuclease protection analysis, can be obtained from as few as 2 x 10(6) cells, making this method particularly suitable for the genetic screening of large numbers of individual, stably transfected cell clones.

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Year:  1989        PMID： 2479289     DOI： 10.1016/0003-2697(89)90435-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Beta-globin locus activation regions: conservation of organization, structure, and function.

Authors:  Q L Li; B Zhou; P Powers; T Enver; G Stamatoyannopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  A Hoechst H33258 agarose plate assay for the estimation of nanogram DNA levels without RNA interference: applications in PCR and in estimations of plasmid and cytoplasmic DNA.

Authors:  M S Rieber; M Rieber
Journal:  Nucleic Acids Res       Date:  1990-04-11       Impact factor: 16.971

3.  Expression of tumor necrosis factor and c-fos genes in peritoneal macrophages of hypothyroid mice.

Authors:  W K Liu
Journal:  Inflammation       Date:  1993-04       Impact factor: 4.092

4.  Serum factors can modulate the developmental clock of gamma- to beta-globin gene switching in somatic cell hybrids.

Authors:  G Zitnik; Q Li; G Stamatoyannopoulos; T Papayannopoulou
Journal:  Mol Cell Biol       Date:  1993-08       Impact factor: 4.272

5.  Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes.

Authors:  G Zitnik; P Hines; G Stamatoyannopoulos; T Papayannopoulou
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

6.  Position independence and proper developmental control of gamma-globin gene expression require both a 5' locus control region and a downstream sequence element.

Authors:  Q Li; J A Stamatoyannopoulos
Journal:  Mol Cell Biol       Date:  1994-09       Impact factor: 4.272

7.  Nuclear translocation of a maternal CCAAT factor at the start of gastrulation activates Xenopus GATA-2 transcription.

Authors:  A C Brewer; M J Guille; D J Fear; G A Partington; R K Patient
Journal:  EMBO J       Date:  1995-02-15       Impact factor: 11.598

8.  GATA1 and YY1 are developmental repressors of the human epsilon-globin gene.

Authors:  N Raich; C H Clegg; J Grofti; P H Roméo; G Stamatoyannopoulos
Journal:  EMBO J       Date:  1995-02-15       Impact factor: 11.598

9.  Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture.

Authors:  Monica Visnieski Alcantara; Stenio Perdigão Fragoso; Gisele Fernanda Assine Picchi
Journal:  Mem Inst Oswaldo Cruz       Date:  2014-06-06       Impact factor: 2.743
  9 in total

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