Literature DB >> 24791981

GeLC-MS/MS analysis of complex protein mixtures.

Monika Dzieciatkowska1, Ryan Hill, Kirk C Hansen.   

Abstract

Discovery-based proteomics has found its place in nearly every facet of biological research. A key objective of this approach is to maximize sequence coverage for proteins across a wide concentration range. Fractionating samples at the protein level is one of the most common ways to circumvent challenges due to sample complexity and improve proteome coverage. Of the available methods, one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by liquid chromatography-tandem mass spectrometry (GeLC-MS/MS) is a robust and reproducible method for qualitative and quantitative proteomic analysis. Here we describe a general GeLC-MS/MS protocol and include technical advice and outline caveats to increase the probability of a successful analysis.

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Year:  2014        PMID： 24791981      PMCID： PMC4416643          DOI： 10.1007/978-1-4939-0685-7_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  28 in total

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