Literature DB >> 24789191

Development and clinical evaluation of sdaA loop-mediated isothermal amplification assay for detection of Mycobacterium tuberculosis with an approach to prevent carryover contamination.

Manoj Nimesh1, Deepali Joon2, Mandira Varma-Basil3, Daman Saluja4.   

Abstract

A rapid and sensitive loop-mediated isothermal amplification assay for the sdaA gene of Mycobacterium tuberculosis was developed using a dUTP-uracil-N-glycosylase (dUTP-UNG) strategy to prevent carryover contamination. Evaluation of the assay using clinical specimens (n = 648) showed high specificity (97.2%) and sensitivity (100%), demonstrating its potential as a diagnostic test for tuberculosis, especially in resource-limited settings.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 24789191      PMCID： PMC4097723          DOI： 10.1128/JCM.00907-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Loop-mediated isothermal amplification of DNA.

Authors:  T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

2.  Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.

Authors:  Soumitesh Chakravorty; Jaya Sivaswami Tyagi
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

3.  Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients.

Authors:  Basu Dev Pandey; Ajay Poudel; Tomoko Yoda; Aki Tamaru; Naozumi Oda; Yukari Fukushima; Binod Lekhak; Basista Risal; Bishnu Acharya; Bishwa Sapkota; Chie Nakajima; Tooru Taniguchi; Benjawan Phetsuksiri; Yasuhiko Suzuki
Journal:  J Med Microbiol       Date:  2008-04       Impact factor: 2.472

4.  A rapid loop-mediated isothermal amplification assay targeting hspX for the detection of Mycobacterium tuberculosis complex.

Authors:  Aixiao Bi; Chie Nakajima; Yukari Fukushima; Aki Tamaru; Isamu Sugawara; Akio Kimura; Ryuji Kawahara; Zhongyi Hu; Yasuhiko Suzuki
Journal:  Jpn J Infect Dis       Date:  2012       Impact factor: 1.362

Review 5.  Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.

Authors:  Linda M Parsons; Akos Somoskövi; Cristina Gutierrez; Evan Lee; C N Paramasivan; Alash'le Abimiku; Steven Spector; Giorgio Roscigno; John Nkengasong
Journal:  Clin Microbiol Rev       Date:  2011-04       Impact factor: 26.132

Review 6.  Use of loop-mediated isothermal amplification of DNA for the rapid detection of Mycobacterium tuberculosis in clinical specimens.

Authors:  I K Neonakis; D A Spandidos; E Petinaki
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-02-18       Impact factor: 3.267

7.  Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples.

Authors:  Tomotada Iwamoto; Toshiaki Sonobe; Kozaburo Hayashi
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

8.  A novel and more sensitive loop-mediated isothermal amplification assay targeting IS6110 for detection of Mycobacterium tuberculosis complex.

Authors:  Ehsan Aryan; Manoochehr Makvandi; Ahmad Farajzadeh; Kris Huygen; Pablo Bifani; Seyed-Latif Mousavi; Abolfazl Fateh; Abbass Jelodar; Mohammad-Mehdi Gouya; Marta Romano
Journal:  Microbiol Res       Date:  2009-06-10       Impact factor: 5.415

9.  Use of visual loop-mediated isotheral amplification of rimM sequence for rapid detection of Mycobacterium tuberculosis and Mycobacterium bovis.

Authors:  Ru-Yi Zhu; Kou-Xing Zhang; Ming-Qiu Zhao; Ya-Hong Liu; Yun-Yi Xu; Chun-Mei Ju; Bing Li; Jin-Ding Chen
Journal:  J Microbiol Methods       Date:  2009-07-17       Impact factor: 2.363

10.  Comparative study of diagnostic accuracy of established PCR assays and in-house developed sdaA PCR method for detection of Mycobacterium tuberculosis in symptomatic patients with pulmonary tuberculosis.

Authors:  Manoj Nimesh; Deepali Joon; Anil Kumar Pathak; Daman Saluja
Journal:  J Infect       Date:  2013-07-16       Impact factor: 6.072
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  4 in total

1.  The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification (LAMP) Assay for Tuberculosis Diagnosis in Adults with Chronic Cough in Malawi.

Authors:  Marriott Nliwasa; Peter MacPherson; Palesa Chisala; Mercy Kamdolozi; McEwen Khundi; Kruger Kaswaswa; Mphatso Mwapasa; Chisomo Msefula; Hojoon Sohn; Clare Flach; Elizabeth L Corbett
Journal:  PLoS One       Date:  2016-05-12       Impact factor: 3.240

Review 2.  Rapid molecular assays for detection of tuberculosis.

Authors:  Rkia Eddabra; Hassan Ait Benhassou
Journal:  Pneumonia (Nathan)       Date:  2018-05-25

3.  Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples.

Authors:  Chao Zhang; Tingting Zheng; Hua Wang; Wei Chen; Xiaoye Huang; Jianqi Liang; Liping Qiu; Da Han; Weihong Tan
Journal:  Anal Chem       Date:  2021-02-11       Impact factor: 6.986

Review 4.  Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis.

Authors:  Kenjiro Nagai; Nobuyuki Horita; Masaki Yamamoto; Toshinori Tsukahara; Hideyuki Nagakura; Ken Tashiro; Yuji Shibata; Hiroki Watanabe; Kentaro Nakashima; Ryota Ushio; Misako Ikeda; Atsuya Narita; Akinori Kanai; Takashi Sato; Takeshi Kaneko
Journal:  Sci Rep       Date:  2016-12-13       Impact factor: 4.379
  4 in total

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