| Literature DB >> 18349362 |
Basu Dev Pandey1,2, Ajay Poudel3, Tomoko Yoda4, Aki Tamaru4, Naozumi Oda5, Yukari Fukushima6, Binod Lekhak3, Basista Risal3, Bishnu Acharya1, Bishwa Sapkota7, Chie Nakajima6, Tooru Taniguchi8, Benjawan Phetsuksiri9, Yasuhiko Suzuki6.
Abstract
A number of nucleic acid amplification assays (NAAs) have been employed to detect tubercle bacilli in clinical specimens for tuberculosis (TB) diagnosis. Among these, loop-mediated isothermal amplification (LAMP) is an NAA possessing superior isothermal reaction characteristics. In the present study, a set of six specific primers targeting the Mycobacterium tuberculosis 16S rRNA gene with high sensitivity was selected and a LAMP system (MTB-LAMP) was developed. Using this system, a total of 200 sputum samples from Nepalese patients were investigated. The sensitivity of MTB-LAMP in culture-positive samples was 100 % (96/96), and the specificity in culture-negative samples was 94.2 % (98/104, 95 % confidence interval 90.5-97.9 %). The positive and negative predictive values of MTB-LAMP were 94.1 and 100 %, respectively. These results indicate that this MTB-LAMP method may prove to be a powerful tool for the early diagnosis of TB.Entities:
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Year: 2008 PMID： 18349362 DOI： 10.1099/jmm.0.47499-0
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472