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Literature DB >> 2477795

Nuclear and cytoplasmic sites for anti-sense control.

Abstract

In transgenic tobacco, anti-sense control of the chimaeric bar (bialophos resistance) gene is manifested by a reduced bar transcript level and a reduced synthesis of bar translational product (PAT, phosphinothricin acetyl transferase) per transcript (1). This study shows that the anti-bar gene controls the transcript level and the translation efficiency of the bar mRNA through independent events and at different cellular sites. On the basis of these results a model for the mechanism of anti-sense control is proposed.

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Year: 1989 PMID： 2477795 PMCID： PMC334800 DOI： 10.1093/nar/17.18.7203

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

Review 1. Antisense genes in plants: an overview.

Authors: A R van der Krol; J N Mol; A R Stuitje
Journal: Gene Date: 1988-12-10 Impact factor: 3.688

2. Both RNA level and translation efficiency are reduced by anti-sense RNA in transgenic tobacco.

Authors: M Cornelissen; M Vandewiele
Journal: Nucleic Acids Res Date: 1989-02-11 Impact factor: 16.971

3. Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

Authors: D A Melton
Journal: Proc Natl Acad Sci U S A Date: 1985-01 Impact factor: 11.205

4. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium.

Authors: T E Crowley; W Nellen; R H Gomer; R A Firtel
Journal: Cell Date: 1985-12 Impact factor: 41.582

5. Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA.

Authors: S K Kim; B J Wold
Journal: Cell Date: 1985-08 Impact factor: 41.582

6. Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

Authors: M D Block; J Botterman; M Vandewiele; J Dockx; C Thoen; V Gosselé; N R Movva; C Thompson; M V Montagu; J Leemans
Journal: EMBO J Date: 1987-09 Impact factor: 11.598

6 in total

13 in total

1. High-efficiency silencing of a beta-glucuronidase gene in rice is correlated with repetitive transgene structure but is independent of DNA methylation.

Authors: M B Wang; P M Waterhouse
Journal: Plant Mol Biol Date: 2000-05 Impact factor: 4.076

Review 2. RNA degradation and models for post-transcriptional gene-silencing.

Authors: F Meins
Journal: Plant Mol Biol Date: 2000-06 Impact factor: 4.076

3. Parameters affecting the activity of antisense RNA sequences in tobacco protoplasts.

Authors: F Guerineau; R Waugh; J W Brown
Journal: Plant Cell Rep Date: 1994-09 Impact factor: 4.570

4. Loss of tumorigenicity of rat glioblastoma directed by episome-based antisense cDNA transcription of insulin-like growth factor I.

Authors: J Trojan; B K Blossey; T R Johnson; S D Rudin; M Tykocinski; J Ilan; J Ilan
Journal: Proc Natl Acad Sci U S A Date: 1992-06-01 Impact factor: 11.205

Nuclear and cytoplasmic sites for anti-sense control.

Review 1. Antisense genes in plants: an overview.

2. Both RNA level and translation efficiency are reduced by anti-sense RNA in transgenic tobacco.

3. Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

4. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium.

5. Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA.

6. Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

1. High-efficiency silencing of a beta-glucuronidase gene in rice is correlated with repetitive transgene structure but is independent of DNA methylation.

Review 2. RNA degradation and models for post-transcriptional gene-silencing.

3. Parameters affecting the activity of antisense RNA sequences in tobacco protoplasts.

4. Loss of tumorigenicity of rat glioblastoma directed by episome-based antisense cDNA transcription of insulin-like growth factor I.

Review 5. Antisense and ribozyme constructs in transgenic animals.

6. Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts.

7. cry IA(b) transcript formation in tobacco is inefficient.

8. Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.

9. Conditional inhibition of beta-glucuronidase expression by antisense gene fragments in petunia protoplasts.

Review 10. Antisense RNA: function and fate of duplex RNA in cells of higher eukaryotes.