Literature DB >> 2476418

Simultaneous application of in situ DNA hybridization and immunohistochemistry on one tissue section.

C M Van der Loos1, H H Volkers, R Rook, F M Van den Berg, H J Houthoff.   

Abstract

Combined application of a non-radioactive in situ DNA hybridization procedure and the immunoperoxidase technique on one tissue section is described. Of six potential protocols, only one proved to be successful. First, the immunohistochemical procedure including visualization of enzyme activity is performed; the in situ DNA hybridization protocol is then applied. Using this protocol, several antigens, detected with monoclonal antibodies, and target DNAs, detected by using biotinylated human cytomegalovirus or human papilloma virus type 16 DNA probes, could be distinguished by their peroxidase activity (brown precipitate) and alkaline phosphatase activity (purple-blue precipitate) respectively. The method allows immunophenotyping of virus-infected cells as well as simultaneous visualization of two viral parameters. This technique has important implications for research and diagnostic purposes.

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Year:  1989        PMID： 2476418     DOI： 10.1007/bf01757180

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  22 in total

1.  In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes.

Authors:  H Hoefler; H Childers; M R Montminy; R M Lechan; R H Goodman; H J Wolfe
Journal:  Histochem J       Date:  1986 Nov-Dec

2.  Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy.

Authors:  S Koenig; H E Gendelman; J M Orenstein; M C Dal Canto; G H Pezeshkpour; M Yungbluth; F Janotta; A Aksamit; M A Martin; A S Fauci
Journal:  Science       Date:  1986-09-05       Impact factor: 47.728

3.  Cytomegalovirus detection by nonisotopic in situ DNA hybridization and viral antigen immunostaining using a two-color technique.

Authors:  R A Wolber; R V Lloyd
Journal:  Hum Pathol       Date:  1988-06       Impact factor: 3.466

4.  Use of commercially available monoclonal antibodies for immunoenzyme double staining.

Authors:  C M van der Loos; J J van den Oord; P K Das; H J Houthoff
Journal:  Histochem J       Date:  1988-08

5.  Combination of immunocytochemistry and in situ hybridization in the same tissue section of rat pituitary.

Authors:  B D Shivers; R E Harlan; D W Pfaff; B S Schachter
Journal:  J Histochem Cytochem       Date:  1986-01       Impact factor: 2.479

6.  Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis.

Authors:  F M van den Berg; M Jiwa; R Rook; J L Geelen
Journal:  J Gen Virol       Date:  1988-03       Impact factor: 3.891

7.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

8.  Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry.

Authors:  A S De Jong; M Van Kessel-van Vark; A K Raap
Journal:  Histochem J       Date:  1985-10

9.  Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors:  J J Leary; D J Brigati; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

10.  Paired indirect immunoenzyme staining with primary antibodies from the same species. Application of horseradish peroxidase and alkaline phosphatase as sequential labels.

Authors:  K Valnes; P Brandtzaeg
Journal:  Histochem J       Date:  1984-05
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  8 in total

1.  Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas.

Authors:  M Denijn; R A De Weger; A D Van Mansfeld; J A van Unnik; C J Lips
Journal:  Histochemistry       Date:  1992

2.  Simultaneous in situ genotyping and phenotyping of human papillomavirus cervical lesions: comparative sensitivity and specificity.

Authors:  A K Graham; C S Herrington; O McGee J
Journal:  J Clin Pathol       Date:  1991-02       Impact factor: 3.411

Review 3.  Practical suggestions for successful immunoenzyme double-staining experiments.

Authors:  C M van der Loos; A E Becker; J J van den Oord
Journal:  Histochem J       Date:  1993-01

4.  Interphase cytogenetics in paraffin sections of lung tumors by non-isotopic in situ hybridization. Mapping genotype/phenotype heterogeneity.

Authors:  S Y Kim; J S Lee; J Y Ro; M L Gay; W K Hong; W N Hittelman
Journal:  Am J Pathol       Date:  1993-01       Impact factor: 4.307

5.  Detection of different developmental stages of malaria parasites by non-radioactive DNA in situ hybridization.

Authors:  F M Van den Berg; P J Van Amstel; C J Janse; J F Meis; B Mons
Journal:  Histochem J       Date:  1991-03

Review 6.  Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors:  E J Speel; F C Ramaekers; A H Hopman
Journal:  Histochem J       Date:  1995-11

7.  A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens.

Authors:  U Müller-Ladner; J Kriegsmann; R E Gay; S Gay
Journal:  Histochem J       Date:  1996-02

8.  Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isotopic, non-fluorescent probes: technical considerations and biological implications.

Authors:  K Dhingra; A Sahin; J Supak; S Y Kim; G Hortobagyi; W N Hittelman
Journal:  Breast Cancer Res Treat       Date:  1992       Impact factor: 4.872
  8 in total

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