Literature DB >> 1618637

Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas.

M Denijn1, R A De Weger, A D Van Mansfeld, J A van Unnik, C J Lips.   

Abstract

We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe--hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.

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Year:  1992        PMID： 1618637     DOI： 10.1007/bf00271279

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  18 in total

1.  In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes.

Authors:  H Hoefler; H Childers; M R Montminy; R M Lechan; R H Goodman; H J Wolfe
Journal:  Histochem J       Date:  1986 Nov-Dec

2.  Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

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Journal:  J Histochem Cytochem       Date:  1978-08       Impact factor: 2.479

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Authors:  B D Shivers; R E Harlan; D W Pfaff; B S Schachter
Journal:  J Histochem Cytochem       Date:  1986-01       Impact factor: 2.479

4.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

5.  Sequence of the human insulin gene.

Authors:  G I Bell; R L Pictet; W J Rutter; B Cordell; E Tischer; H M Goodman
Journal:  Nature       Date:  1980-03-06       Impact factor: 49.962

6.  Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients.

Authors:  G J Cooper; A C Willis; A Clark; R C Turner; R B Sim; K B Reid
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

7.  Localisation of islet amyloid peptide in lipofuscin bodies and secretory granules of human B-cells and in islets of type-2 diabetic subjects.

Authors:  A Clark; C A Edwards; L R Ostle; R Sutton; J B Rothbard; J F Morris; R C Turner
Journal:  Cell Tissue Res       Date:  1989-07       Impact factor: 5.249

8.  A novel peptide in the calcitonin gene related peptide family as an amyloid fibril protein in the endocrine pancreas.

Authors:  P Westermark; C Wernstedt; E Wilander; K Sletten
Journal:  Biochem Biophys Res Commun       Date:  1986-11-14       Impact factor: 3.575

9.  Conservation of the sequence of islet amyloid polypeptide in five mammals is consistent with its putative role as an islet hormone.

Authors:  M Nishi; S J Chan; S Nagamatsu; G I Bell; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

10.  Rat amylin: cloning and tissue-specific expression in pancreatic islets.

Authors:  J D Leffert; C B Newgard; H Okamoto; J L Milburn; K L Luskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205
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  2 in total

1.  Immunoreactivity and expression of amylin in gastroenteropancreatic endocrine tumors.

Authors:  R Eissele; C Neuhaus; M E Trautmann; A Funk; R Arnold; H Höfler
Journal:  Am J Pathol       Date:  1993-07       Impact factor: 4.307

2.  Islet amyloid polypeptide gene expression in the endocrine pancreas of the rat: a combined in situ hybridization and immunocytochemical study.

Authors:  H Mulder; A C Lindh; F Sundler
Journal:  Cell Tissue Res       Date:  1993-12       Impact factor: 5.249
  2 in total

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