Literature DB >> 2832532

Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis.

F M van den Berg1, M Jiwa, R Rook, J L Geelen.   

Abstract

High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.

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Year:  1988        PMID： 2832532     DOI： 10.1099/0022-1317-69-3-699

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  8 in total

1.  Detection of different developmental stages of malaria parasites by non-radioactive DNA in situ hybridization.

Authors:  F M Van den Berg; P J Van Amstel; C J Janse; J F Meis; B Mons
Journal:  Histochem J       Date:  1991-03

2.  Implausibility of an aetiological association between cytomegalovirus and Kaposi's sarcoma shown by four techniques.

Authors:  F Van den Berg; M Schipper; M Jiwa; R Rook; F Van de Rijke; B Tigges
Journal:  J Clin Pathol       Date:  1989-02       Impact factor: 3.411

3.  On-line sorting of human chromosomes by centromeric index, and identification of sorted populations by GTG-banding and fluorescent in situ hybridization.

Authors:  G A Boschman; W Rens; E Manders; C van Oven; G W Barendsen; J A Aten
Journal:  Hum Genet       Date:  1990-06       Impact factor: 4.132

4.  Simultaneous application of in situ DNA hybridization and immunohistochemistry on one tissue section.

Authors:  C M Van der Loos; H H Volkers; R Rook; F M Van den Berg; H J Houthoff
Journal:  Histochem J       Date:  1989-05

5.  Physical mapping and field inversion gel electrophoresis of Amsacta moorei entomopoxvirus DNA.

Authors:  R L Hall; W F Hink
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

6.  Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C.

Authors:  K Hirai; M Yamada; Y Arao; S Kato; S Nii
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

7.  Demonstration of a Sandhoff disease-associated autosomal 50-kb deletion by field inversion gel electrophoresis.

Authors:  H Bikker; F M van den Berg; R A Wolterman; J J de Vijlder; P A Bolhuis
Journal:  Hum Genet       Date:  1989-02       Impact factor: 4.132

8.  Detection of Campylobacter pylori in stomach tissue by DNA in situ hybridisation.

Authors:  F M Van den Berg; H Zijlmans; W Langenberg; E Rauws; M Schipper
Journal:  J Clin Pathol       Date:  1989-09       Impact factor: 3.411
  8 in total

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