INTRODUCTION AND HYPOTHESIS: The rs1800255, COL3A1 2209 G>A polymorphism in the alpha 1 chain of collagen type III has been associated with an increased risk of pelvic organ prolapse (POP). In one of our previous studies however, polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) misdiagnosed rs1800255, COL3A1 2209 G>A in 6 % of cases. The high-resolution melting (HRM) analysis on the contrary obtained a 100 % accordance for this specific polymorphism and was used in the present study to validate this risk factor for POP. METHODS: In this case-control study, women with and without symptoms of POP were included and compared. DNA was extracted from blood samples. HRM analysis was used to assess for the presence of the homozygous rs1800255. Groups were compared using the Pearson chi-square, Mann-Whitney, and t tests. The discrepancy between HRM and PCR-RFLP results was investigated using PCR-RFLP results available from our previous study. RESULTS: The study included 354 women: 272 patients with POP and 82 controls; 18 (7 %) cases versus 3 (4 %) controls had a homozygous rs1800255, COL3A1 2209 G>A polymorphism (odds ratio 1.9, 95 % confidence interval 0.5-6.9, compared to the wild type), and thus no association between POP and the homozygous polymorphism could be demonstrated. A discrepancy between HRM and PCR-RFLP results was found in 8 % of the samples. CONCLUSIONS: The previously found statistically significant association between the rs1800255, COL3A1 2209 G>A polymorphism as measured with PCR-RFLP and POP could no longer be demonstrated. This raises concerns regarding the results of other association studies using PCR-RFLP.
INTRODUCTION AND HYPOTHESIS: The rs1800255, COL3A1 2209 G>A polymorphism in the alpha 1 chain of collagen type III has been associated with an increased risk of pelvic organ prolapse (POP). In one of our previous studies however, polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) misdiagnosed rs1800255, COL3A1 2209 G>A in 6 % of cases. The high-resolution melting (HRM) analysis on the contrary obtained a 100 % accordance for this specific polymorphism and was used in the present study to validate this risk factor for POP. METHODS: In this case-control study, women with and without symptoms of POP were included and compared. DNA was extracted from blood samples. HRM analysis was used to assess for the presence of the homozygous rs1800255. Groups were compared using the Pearson chi-square, Mann-Whitney, and t tests. The discrepancy between HRM and PCR-RFLP results was investigated using PCR-RFLP results available from our previous study. RESULTS: The study included 354 women: 272 patients with POP and 82 controls; 18 (7 %) cases versus 3 (4 %) controls had a homozygous rs1800255, COL3A1 2209 G>A polymorphism (odds ratio 1.9, 95 % confidence interval 0.5-6.9, compared to the wild type), and thus no association between POP and the homozygous polymorphism could be demonstrated. A discrepancy between HRM and PCR-RFLP results was found in 8 % of the samples. CONCLUSIONS: The previously found statistically significant association between the rs1800255, COL3A1 2209 G>A polymorphism as measured with PCR-RFLP and POP could no longer be demonstrated. This raises concerns regarding the results of other association studies using PCR-RFLP.
Authors: Gunhilde M Buchsbaum; Erin E Duecy; Lindsey A Kerr; Li-Shan Huang; MaryAnn Perevich; David S Guzick Journal: Obstet Gynecol Date: 2006-12 Impact factor: 7.661
Authors: Kirsten B Kluivers; Jeroen R Dijkstra; Jan C M Hendriks; Sabrina L Lince; Mark E Vierhout; Léon C L van Kempen Journal: Int Urogynecol J Pelvic Floor Dysfunct Date: 2009-05-15
Authors: Myung Jae Jeon; Sue Min Chung; Jong Rak Choi; Hyun Joo Jung; Sei Kwang Kim; Sang Wook Bai Journal: J Urol Date: 2009-01-18 Impact factor: 7.450
Authors: Fernando Henrique Teixeira; César Eduardo Fernandes; Ricardo Peres do Souto; Emerson de Oliveira Journal: Int Urogynecol J Date: 2019-04-30 Impact factor: 2.894
Authors: Rufus Cartwright; Anna C Kirby; Kari A O Tikkinen; Altaf Mangera; Gans Thiagamoorthy; Prabhakar Rajan; Jori Pesonen; Chris Ambrose; Juan Gonzalez-Maffe; Phillip Bennett; Tom Palmer; Andrew Walley; Marjo-Riitta Järvelin; Chris Chapple; Vik Khullar Journal: Am J Obstet Gynecol Date: 2014-08-08 Impact factor: 8.661
Authors: Kristina Allen-Brady; John W F Chua; Romana Cuffolo; Marianne Koch; Felice Sorrentino; Rufus Cartwright Journal: Int Urogynecol J Date: 2021-04-24 Impact factor: 1.932