Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A two-armed lanthanoid-chelating paramagnetic NMR probe linked to proteins via thioether linkages.

Literature DB >> 24737492

A two-armed lanthanoid-chelating paramagnetic NMR probe linked to proteins via thioether linkages.

Wei-Min Liu¹, Simon P Skinner, Monika Timmer, Anneloes Blok, Mathias A S Hass, Dmitri V Filippov, Mark Overhand, Marcellus Ubbink.

Abstract

Paramagnetic NMR probes provide valuable long-range structural information on proteins and protein complexes. A new, stable, two-armed lanthanoid probe is reported that can be attached to a protein site-specifically via chemically inert thioether linkages.

Entities: Chemical

Keywords: lanthanides; paramagnetic NMR; protein modification; protein structures; pseudocontact shift

Mesh：

Substances：

Year: 2014 PMID： 24737492 DOI： 10.1002/chem.201400257

Source DB: PubMed Journal: Chemistry ISSN： 0947-6539 Impact factor: 5.236

Keyword Cloud
Cited

8 in total

1. Delicate conformational balance of the redox enzyme cytochrome P450cam.

Authors: Simon P Skinner; Wei-Min Liu; Yoshitaka Hiruma; Monika Timmer; Anneloes Blok; Mathias A S Hass; Marcellus Ubbink
Journal: Proc Natl Acad Sci U S A Date: 2015-06-30 Impact factor: 11.205

2. Paramagnetic relaxation enhancement of membrane proteins by incorporation of the metal-chelating unnatural amino acid 2-amino-3-(8-hydroxyquinolin-3-yl)propanoic acid (HQA).

Authors: Sang Ho Park; Vivian S Wang; Jasmina Radoicic; Anna A De Angelis; Sabrina Berkamp; Stanley J Opella
Journal: J Biomol NMR Date: 2014-11-28 Impact factor: 2.835

A two-armed lanthanoid-chelating paramagnetic NMR probe linked to proteins via thioether linkages.

1. Delicate conformational balance of the redox enzyme cytochrome P450cam.

2. Paramagnetic relaxation enhancement of membrane proteins by incorporation of the metal-chelating unnatural amino acid 2-amino-3-(8-hydroxyquinolin-3-yl)propanoic acid (HQA).

Review 3. Paramagnetic Chemical Probes for Studying Biological Macromolecules.

4. A nucleotide-independent cyclic nitroxide label for monitoring segmental motions in nucleic acids.

5. Integral membrane protein structure determination using pseudocontact shifts.

6. Compact, hydrophilic, lanthanide-binding tags for paramagnetic NMR spectroscopy.

7. NMR pseudocontact shifts in a symmetric protein homotrimer.

8. Paramagnetic Ligand Tagging To Identify Protein Binding Sites.