Literature DB >> 2473220

Epitope mapping of monoclonal antibodies against human immunodeficiency virus type 1 structural proteins by using peptides.

M Niedrig1, J Hinkula, W Weigelt, J L'age-Stehr, G Pauli, J Rosen, B Wahren.   

Abstract

Murine monoclonal antibodies directed against the structural proteins p17 and p24 of human immunodeficiency virus type 1 were investigated in an epitope mapping system. Overlapping peptides consisting of 15 amino acids of the p17 and p24 protein, respectively, were used as competitors in an enzyme-linked immunosorbent assay. Three different immunogenic regions (A, B, and C) could be defined, one on p17 and two on p24. Twenty monoclonal antibodies reacted with the human immunodeficiency virus type 1 peptides of region B, although differences in the reactivity of these antibodies with human immunodeficiency virus type 2 and simian immunodeficiency virus strain mac were detectable. Recognized epitopes were characterized by computer analysis as described by T.P. Hopp and K.R. Woods (Proc. Natl. Acad. Sci. USA 78:3824-3828, 1981) and P.Y. Chou and G.D. Fasman (Biochemistry 13:222-245, 1974).

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Year:  1989        PMID： 2473220      PMCID： PMC250932     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  18 in total

1.  Persistent HIV antigenaemia and decline of HIV core antibodies associated with transition to AIDS.

Authors:  J M Lange; D A Paul; H G Huisman; F de Wolf; H van den Berg; R A Coutinho; S A Danner; J van der Noordaa; J Goudsmit
Journal:  Br Med J (Clin Res Ed)       Date:  1986-12-06

2.  General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids.

Authors:  R A Houghten
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205

3.  Prediction of antigenic determinants and secondary structures of the major AIDS virus proteins.

Authors:  M J Sternberg; G J Barton; M J Zvelebil; J Cookson; A R Coates
Journal:  FEBS Lett       Date:  1987-06-29       Impact factor: 4.124

4.  Complete nucleotide sequence of the AIDS virus, HTLV-III.

Authors:  L Ratner; W Haseltine; R Patarca; K J Livak; B Starcich; S F Josephs; E R Doran; J A Rafalski; E A Whitehorn; K Baumeister
Journal:  Nature       Date:  1985 Jan 24-30       Impact factor: 49.962

5.  Impaired specific cellular response to HTLV-III before other immune defects in patients with HTLV-III infection.

Authors:  B Wahren; L Morfeldt-Månsson; G Biberfeld; L Moberg; P Ljungman; S Nordlund; U Bredberg-Rådén; A Werner; J Löwer; R Kurth
Journal:  N Engl J Med       Date:  1986-08-07       Impact factor: 91.245

6.  Prediction of protein conformation.

Authors:  P Y Chou; G D Fasman
Journal:  Biochemistry       Date:  1974-01-15       Impact factor: 3.162

7.  Genome organization and transactivation of the human immunodeficiency virus type 2.

Authors:  M Guyader; M Emerman; P Sonigo; F Clavel; L Montagnier; M Alizon
Journal:  Nature       Date:  1987 Apr 16-22       Impact factor: 49.962

8.  Isolation of T-cell tropic HTLV-III-like retrovirus from macaques.

Authors:  M D Daniel; N L Letvin; N W King; M Kannagi; P K Sehgal; R D Hunt; P J Kanki; M Essex; R C Desrosiers
Journal:  Science       Date:  1985-06-07       Impact factor: 47.728

9.  Monoclonal antibodies directed against human immunodeficiency virus (HIV) gag proteins with specificity for conserved epitopes in HIV-1, HIV-2 and simian immunodeficiency virus.

Authors:  M Niedrig; J P Rabanus; J L'Age Stehr; H R Gelderblom; G Pauli
Journal:  J Gen Virol       Date:  1988-08       Impact factor: 3.891

10.  Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins.

Authors:  H R Gelderblom; E H Hausmann; M Ozel; G Pauli; M A Koch
Journal:  Virology       Date:  1987-01       Impact factor: 3.616
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  14 in total

1.  Expression of nef, vpu, CA and CD4 during the infection of lymphoid and monocytic cell lines with HIV-1.

Authors:  T Schneider; P Hildebrandt; K Rokos; U Schubert; W Rönspeck; C Grund; A Beck; R Blesken; G Kulins; H Oldenburg
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

2.  Infection of the human monocytic cell line Mono Mac6 with human immunodeficiency virus types 1 and 2 results in long-term production of virus variants with increased cytopathogenicity for CD4+ T cells.

Authors:  J L'age-Stehr; M Niedrig; H R Gelderblom; J W Sim-Brandenburg; M Urban-Schriefer; E P Rieber; J G Haas; G Riethmüller; H W Ziegler-Heitbrock
Journal:  J Virol       Date:  1990-08       Impact factor: 5.103

3.  Primitive hematopoietic cells resist HIV-1 infection via p21.

Authors:  Jielin Zhang; David T Scadden; Clyde S Crumpacker
Journal:  J Clin Invest       Date:  2007-02       Impact factor: 14.808

4.  Inhibition of early and late events of the HIV-1 replication cycle by cytoplasmic Fab intrabodies against the matrix protein, p17.

Authors:  R Levin; A M Mhashilkar; T Dorfman; A Bukovsky; C Zani; J Bagley; J Hinkula; M Niedrig; J Albert; B Wahren; H G Göttlinger; W A Marasco
Journal:  Mol Med       Date:  1997-02       Impact factor: 6.354

5.  Linear B-cell epitopes of the major core protein of human immunodeficiency virus types 1 and 2.

Authors:  B Janvier; P Archinard; B Mandrand; A Goudeau; F Barin
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

6.  Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein, p17gag: identification of epitopes exposed at the surfaces of infected cells.

Authors:  F Shang; H Huang; K Revesz; H C Chen; R Herz; A Pinter
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

7.  Characterization of murine monoclonal antibodies directed against the core proteins of human immunodeficiency virus types 1 and 2.

Authors:  M Niedrig; J Hinkula; H P Harthus; M Bröker; L Hopp; G Pauli; B Wahren
Journal:  J Virol       Date:  1991-08       Impact factor: 5.103

8.  Epitope analysis of capsid and matrix proteins of North American ovine lentivirus field isolates.

Authors:  K A Marcom; L D Pearson; C S Chung; J M Poulson; J C DeMartini
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

9.  Selection of recombinant, library-derived antibody fragments against p24 for human immunodeficiency virus type 1 diagnostics.

Authors:  H J de Haard; B Kazemier; M J Koolen; L J Nijholt; R H Meloen; B van Gemen; H R Hoogenboom; J W Arends
Journal:  Clin Diagn Lab Immunol       Date:  1998-09

10.  A large deletion in the matrix domain of the human immunodeficiency virus gag gene redirects virus particle assembly from the plasma membrane to the endoplasmic reticulum.

Authors:  M Fäcke; A Janetzko; R L Shoeman; H G Kräusslich
Journal:  J Virol       Date:  1993-08       Impact factor: 5.103
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