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Literature DB >> 24718797

Light-inducible gene regulation with engineered zinc finger proteins.

Abstract

The coupling of light-inducible protein-protein interactions with gene regulation systems has enabled the control of gene expression with light. In particular, heterodimer protein pairs from plants can be used to engineer a gene regulation system in mammalian cells that is reversible, repeatable, tunable, controllable in a spatiotemporal manner, and targetable to any DNA sequence. This system, Light-Inducible Transcription using Engineered Zinc finger proteins (LITEZ), is based on the blue light-induced interaction of GIGANTEA and the LOV domain of FKF1 that drives the localization of a transcriptional activator to the DNA-binding site of a highly customizable engineered zinc finger protein. This chapter provides methods for modifying LITEZ to target new DNA sequences, engineering a programmable LED array to illuminate cell cultures, and using the modified LITEZ system to achieve spatiotemporal control of transgene expression in mammalian cells.

Entities: CellLine Chemical Disease Gene Species

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Year: 2014 PMID： 24718797 PMCID： PMC4082823 DOI： 10.1007/978-1-4939-0470-9_7

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

43 in total

1. From dusk till dawn: one-plasmid systems for light-regulated gene expression.

Authors: Robert Ohlendorf; Roee R Vidavski; Avigdor Eldar; Keith Moffat; Andreas Möglich
Journal: J Mol Biol Date: 2012-01-08 Impact factor: 5.469

2. Spatiotemporal control of gene expression by a light-switchable transgene system.

Authors: Xue Wang; Xianjun Chen; Yi Yang
Journal: Nat Methods Date: 2012-02-12 Impact factor: 28.547

3. Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system.

Authors: Stacey Thibodeau-Beganny; Morgan L Maeder; J Keith Joung
Journal: Methods Mol Biol Date: 2010

4. Benzoate X receptor zinc-finger gene switches for drug-inducible regulation of transcription.

Authors: L J Schwimmer; B Gonzalez; C F Barbas
Journal: Gene Ther Date: 2011-07-28 Impact factor: 5.250

5. Engineering zinc finger nucleases for targeted mutagenesis of zebrafish.

Authors: Jeffry D Sander; J-R Joanna Yeh; Randall T Peterson; J Keith Joung
Journal: Methods Cell Biol Date: 2011 Impact factor: 1.441

6. Structural basis of photosensitivity in a bacterial light-oxygen-voltage/helix-turn-helix (LOV-HTH) DNA-binding protein.

Authors: Abigail I Nash; Reginald McNulty; Mary Elizabeth Shillito; Trevor E Swartz; Roberto A Bogomolni; Hartmut Luecke; Kevin H Gardner
Journal: Proc Natl Acad Sci U S A Date: 2011-05-23 Impact factor: 11.205

7. The generation of zinc finger proteins by modular assembly.

Authors: Mital S Bhakta; David J Segal
Journal: Methods Mol Biol Date: 2010

8. A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice.

Authors: Haifeng Ye; Marie Daoud-El Baba; Ren-Wang Peng; Martin Fussenegger
Journal: Science Date: 2011-06-24 Impact factor: 47.728

9. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).

Authors: Jeffry D Sander; Elizabeth J Dahlborg; Mathew J Goodwin; Lindsay Cade; Feng Zhang; Daniel Cifuentes; Shaun J Curtin; Jessica S Blackburn; Stacey Thibodeau-Beganny; Yiping Qi; Christopher J Pierick; Ellen Hoffman; Morgan L Maeder; Cyd Khayter; Deepak Reyon; Drena Dobbs; David M Langenau; Robert M Stupar; Antonio J Giraldez; Daniel F Voytas; Randall T Peterson; Jing-Ruey J Yeh; J Keith Joung
Journal: Nat Methods Date: 2010-12-12 Impact factor: 28.547

10. Rapid blue-light-mediated induction of protein interactions in living cells.

Authors: Matthew J Kennedy; Robert M Hughes; Leslie A Peteya; Joel W Schwartz; Michael D Ehlers; Chandra L Tucker
Journal: Nat Methods Date: 2010-10-31 Impact factor: 28.547

10 in total

1. A method to specifically activate the Klotho promoter by using zinc finger proteins constructed from modular building blocks and from naturally engineered Egr1 transcription factor backbone.

Authors: Ci-Di Chen; Melissa A Rudy; Ella Zeldich; Carmela R Abraham
Journal: FASEB J Date: 2020-04-29 Impact factor: 5.191

Review 2. Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools.

Authors: Daria M Shcherbakova; Anton A Shemetov; Andrii A Kaberniuk; Vladislav V Verkhusha
Journal: Annu Rev Biochem Date: 2015-02-20 Impact factor: 23.643

3. An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis.

Authors: Lauren R Polstein; Mark Juhas; Gabi Hanna; Nenad Bursac; Charles A Gersbach
Journal: ACS Synth Biol Date: 2017-09-06 Impact factor: 5.110

4. Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation.

Authors: Nyla Naim; Jeff M Reece; Xuefeng Zhang; Daniel L Altschuler
Journal: Methods Mol Biol Date: 2020

5. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.

Authors: Lauren R Polstein; Charles A Gersbach
Journal: Nat Chem Biol Date: 2015-02-09 Impact factor: 15.040

6. Optogenetic regulation of site-specific subtelomeric DNA methylation.

Authors: Samrat Roy Choudhury; Yi Cui; Anoop Narayanan; David P Gilley; Nazmul Huda; Chiao-Ling Lo; Feng C Zhou; Dinesh Yernool; Joseph Irudayaraj
Journal: Oncotarget Date: 2016-08-02

7. Blue Light-Induced Gene Expression Alterations in Cultured Neurons Are the Result of Phototoxic Interactions with Neuronal Culture Media.

Authors: Corey G Duke; Katherine E Savell; Jennifer J Tuscher; Robert A Phillips; Jeremy J Day
Journal: eNeuro Date: 2020-01-06

Light-inducible gene regulation with engineered zinc finger proteins.

1. From dusk till dawn: one-plasmid systems for light-regulated gene expression.

2. Spatiotemporal control of gene expression by a light-switchable transgene system.

3. Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system.

4. Benzoate X receptor zinc-finger gene switches for drug-inducible regulation of transcription.

5. Engineering zinc finger nucleases for targeted mutagenesis of zebrafish.

6. Structural basis of photosensitivity in a bacterial light-oxygen-voltage/helix-turn-helix (LOV-HTH) DNA-binding protein.

7. The generation of zinc finger proteins by modular assembly.

8. A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice.

9. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).

10. Rapid blue-light-mediated induction of protein interactions in living cells.

1. A method to specifically activate the Klotho promoter by using zinc finger proteins constructed from modular building blocks and from naturally engineered Egr1 transcription factor backbone.

Review 2. Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools.

3. An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis.

4. Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation.

5. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.

6. Optogenetic regulation of site-specific subtelomeric DNA methylation.

7. Blue Light-Induced Gene Expression Alterations in Cultured Neurons Are the Result of Phototoxic Interactions with Neuronal Culture Media.

Review 8. Bringing Light to Transcription: The Optogenetics Repertoire.

9. Optogenetic control of mesenchymal cell fate towards precise bone regeneration.

Review 10. Genetic and epigenetic editing in nervous system .

Review 2. Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools.

Review 8. Bringing Light to Transcription: The Optogenetics Repertoire.

Review 10. Genetic and epigenetic editing in nervous system .

Review 10. Genetic and epigenetic editing in nervous system .