Manjunath Manubolu1, Lavanya Goodla2, Sivajyothi Ravilla3, Jayakumar Thanasekaran4, Paresh Dutta5, Kjell Malmlöf6, Vijayasarathi Reddy Obulum3. 1. Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences (SLU), Box 7011, 750 07 Uppsala, Sweden; Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India. Electronic address: manjunath.manubolu@slu.se. 2. Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India; Natural Products Research Centre, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. 3. Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India. 4. Department of Pharmacology, Taipei Medical University, No. 250, Wu-hsing Street, Taipei 110, Taiwan. 5. Department of Food Science and Food Chemistry, Swedish University of Agricultural Sciences, Uppsala 756 51, Sweden. 6. Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences (SLU), Box 7011, 750 07 Uppsala, Sweden.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Actiniopteris radiata is a herb with great medicinal value and is evaluated for hepatoprotective activity. To investigate the protective effect of ethanolic extract of Actiniopteris radiata (EEAR) on CCl₄ induced oxidative stress in male Wistar albino rats. MATERIALS AND METHODS: EEAR were administered for 8 consecutive weeks to rats. Group I - control; Group II - toxin control (30% CCl₄); Group III and Group IV received EEAR (250 and 500 mg/kg respectively). Antioxidant status in liver were estimated by determining the activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx); as well as by determining the levels of lipid peroxidation (LPO) and reduced glutathione (GSH). In addition, isoenzyme pattern and mRNA expression of the antioxidants were studied. Partial characterization of EEAR was performed by Liquid chromatography-mass spectrometry (LC-MS). RESULTS: CCl₄ induced oxidative stress as evidenced from increase in LPO along with reduction of SOD, CAT, GPx and GSH. Treatment with EEAR (250 and 500 mg/kg) mitigated the CCl₄ induced oxidative stress. An analysis of the isozyme pattern of these antioxidant enzymes revealed variations in SOD2, CAT, GPx2 and GPx3 in CCl₄ treated rats, which were normalized after EEAR treatment. Furthermore, expression of genes for the antioxidant enzymes, were down-regulated by CCl₄ treatment, which were reversed by EEAR. The results of partial characterization of EEAR by LC-MS revealed the presence of rutin and other 7 unknown phenolic derivatives. CONCLUSIONS: These findings suggest the protective effect of EEAR against CCl₄ induced oxidative stress might be attributed to the presence of flavonoids and phenolic compounds.
ETHNOPHARMACOLOGICAL RELEVANCE: Actiniopteris radiata is a herb with great medicinal value and is evaluated for hepatoprotective activity. To investigate the protective effect of ethanolic extract of Actiniopteris radiata (EEAR) on CCl₄ induced oxidative stress in male Wistar albino rats. MATERIALS AND METHODS:EEAR were administered for 8 consecutive weeks to rats. Group I - control; Group II - toxin control (30% CCl₄); Group III and Group IV received EEAR (250 and 500 mg/kg respectively). Antioxidant status in liver were estimated by determining the activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx); as well as by determining the levels of lipid peroxidation (LPO) and reduced glutathione (GSH). In addition, isoenzyme pattern and mRNA expression of the antioxidants were studied. Partial characterization of EEAR was performed by Liquid chromatography-mass spectrometry (LC-MS). RESULTS: CCl₄ induced oxidative stress as evidenced from increase in LPO along with reduction of SOD, CAT, GPx and GSH. Treatment with EEAR (250 and 500 mg/kg) mitigated the CCl₄ induced oxidative stress. An analysis of the isozyme pattern of these antioxidant enzymes revealed variations in SOD2, CAT, GPx2 and GPx3 in CCl₄ treated rats, which were normalized after EEAR treatment. Furthermore, expression of genes for the antioxidant enzymes, were down-regulated by CCl₄ treatment, which were reversed by EEAR. The results of partial characterization of EEAR by LC-MS revealed the presence of rutin and other 7 unknown phenolic derivatives. CONCLUSIONS: These findings suggest the protective effect of EEAR against CCl₄ induced oxidative stress might be attributed to the presence of flavonoids and phenolic compounds.
Authors: Kadry M Sadek; Mohamed A Lebda; Tarek K Abouzed; Sherif M Nasr; Moustafa Shoukry Journal: Metab Brain Dis Date: 2017-06-28 Impact factor: 3.584
Authors: Kadry M Sadek; Mohamed A Lebda; Tarek K Abouzed; Sherif M Nasr; Yasser El-Sayed Journal: Environ Sci Pollut Res Int Date: 2018-09-24 Impact factor: 4.223