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Literature DB >> 2466645

Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels.

Abstract

A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05-0.1 ng protein per band. Total staining time with the new procedure is 75 min.

Entities: Chemical

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Year: 1988 PMID： 2466645 DOI： 10.1002/elps.1150090106

Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535

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Cited

139 in total

1. Glycosylation-site-selective synthesis of N-acetyl-lactosamine repeats in bis-glycosylated human lysozyme.

Authors: R Melcher; A Hillebrand; U Bahr; B Schröder; M Karas; A Hasilik
Journal: Biochem J Date: 2000-06-15 Impact factor: 3.857

2. Purification and characterization of a 200 kDa fructosyllysine-specific binding protein from cell membranes of U937 cells.

Authors: R Salazar; R Brandt; J Kellermann; S Krantz
Journal: Glycoconj J Date: 2000-10 Impact factor: 2.916

3. Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques.

Authors: K Takada; T Hirakawa; H Yokosawa; Y Okawa; H Taguchi; K Ohkawa
Journal: Biochem J Date: 2001-05-15 Impact factor: 3.857

4. Identification of an akinete marker gene in Anabaena variabilis.

Authors: Ruanbao Zhou; C Peter Wolk
Journal: J Bacteriol Date: 2002-05 Impact factor: 3.490

5. Evidence for the formation of a heterotrimeric complex of leukaemia inhibitory factor with its receptor subunits in solution.

Authors: J G Zhang; C M Owczarek; L D Ward; G J Howlett; L J Fabri; B A Roberts; N A Nicola
Journal: Biochem J Date: 1997-08-01 Impact factor: 3.857

10. Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunit a fold similar to the protein in the cell membrane.

Authors: Eva-Maria E Uhlemann; Hannah E Pierson; Robert H Fillingame; Oleg Y Dmitriev
Journal: Protein Sci Date: 2012-01-04 Impact factor: 6.725

Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels.

1. Glycosylation-site-selective synthesis of N-acetyl-lactosamine repeats in bis-glycosylated human lysozyme.

2. Purification and characterization of a 200 kDa fructosyllysine-specific binding protein from cell membranes of U937 cells.

3. Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques.

4. Identification of an akinete marker gene in Anabaena variabilis.

5. Evidence for the formation of a heterotrimeric complex of leukaemia inhibitory factor with its receptor subunits in solution.

6. Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line.

7. Novel DNA-binding proteins in the cyanobacterium Anabaena sp. strain PCC 7120.

8. Transmission electron microscopy comparison of methods for collecting in situ formed enamel pellicle.

9. The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3.

10. Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunit a fold similar to the protein in the cell membrane.