Literature DB >> 11425191

Purification and characterization of a 200 kDa fructosyllysine-specific binding protein from cell membranes of U937 cells.

R Salazar1, R Brandt, J Kellermann, S Krantz.   

Abstract

Amadori-modified proteins are bound by macrophages and monocytes via fructosyllysine-specific receptors. Detergent extracts from U937 cell membranes were used to purify the binding proteins by affinity purification on glycated polylysine coated magnetic beads followed by SDS-PAGE. Two proteins of 200 and 100kDa were isolated. MS-analysis of the 200 kDa protein showed high homologies with cellular myosin heavy chain, type A. Both fructosyllysine specific binding proteins, cellular myosin heavy chain and nucleolin, are glycosylated.

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Year:  2000        PMID： 11425191     DOI： 10.1023/a:1011074705615

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  10 in total

1.  Cellular myosin heavy chain in human leukocytes: isolation of 5' cDNA clones, characterization of the protein, chromosomal localization, and upregulation during myeloid differentiation.

Authors:  L E Toothaker; D A Gonzalez; N Tung; R S Lemons; M M Le Beau; M A Arnaout; L K Clayton; D G Tenen
Journal:  Blood       Date:  1991-10-01       Impact factor: 22.113

2.  Evidence for binding of in vitro glycated low-density lipoproteins by fructosyllysine-specific sites on macrophages and U937 monocyte-like cells.

Authors:  S Krantz; M Michalke; R Brandt; R Salazar; K Hartmann
Journal:  Exp Clin Endocrinol Diabetes       Date:  1997       Impact factor: 2.949

3.  Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels.

Authors:  J Heukeshoven; R Dernick
Journal:  Electrophoresis       Date:  1988-01       Impact factor: 3.535

Review 4.  The nonenzymatic glycation of proteins and nucleic acids, their importance for the development of diabetic complications, possible molecular basis of aging and autoimmunological processes.

Authors:  S Krantz; M Lober; L Henschel
Journal:  Exp Clin Endocrinol       Date:  1986-12

5.  Differential expression of fructosyllysine-specific receptors on monocytes and macrophages and possible pathophysiological significance.

Authors:  R Brandt; C Landmesser; L Vogt; B Hehmke; R Hanschke; J Kasbohm; K Hartmann; B Jäger; S Krantz; D Michaelis
Journal:  Diabetologia       Date:  1996-10       Impact factor: 10.122

6.  Expression of fructosyllysine receptors on human monocytes and monocyte-like cell lines.

Authors:  R Salazar; R Brandt; S Krantz
Journal:  Biochim Biophys Acta       Date:  1995-04-06

7.  Purification and partial amino acid sequencing of a fructosyllysine-specific binding protein from cell membranes of the monocyte-like cell line U937.

Authors:  S Krantz; R Salazar; R Brandt; J Kellermann; F Lottspeich
Journal:  Biochim Biophys Acta       Date:  1995-04-06

8.  Cloning and sequencing of the human nucleolin cDNA.

Authors:  M Srivastava; P J Fleming; H B Pollard; A L Burns
Journal:  FEBS Lett       Date:  1989-06-19       Impact factor: 4.124

9.  Association of a cellular myosin II with anionic phospholipids and the neuronal plasma membrane.

Authors:  D Li; M Miller; P D Chantler
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-01       Impact factor: 11.205

10.  [Blockage of lysine by Mallard's reaction. II. Chemical properties of derivatives N-(deoxy-1-D-fructosyl-1) and N-(deoxy-1-D-lactulosyl-1) lysine].

Authors:  P A Finot; J Mauron
Journal:  Helv Chim Acta       Date:  1972       Impact factor: 2.164
  10 in total
  5 in total

Review 1.  RNA-binding protein nucleolin in disease.

Authors:  Kotb Abdelmohsen; Myriam Gorospe
Journal:  RNA Biol       Date:  2012-05-23       Impact factor: 4.652

2.  Binding of Amadori glucose-modified albumin by the monocytic cell line MonoMac 6 activates protein kinase C epsilon protein tyrosine kinases and the transcription factors AP-1 and NF-kappaB.

Authors:  R Salazar; R Brandt; S Krantz
Journal:  Glycoconj J       Date:  2001-10       Impact factor: 2.916

Review 3.  Amadori-modified glycated serum proteins and accelerated atherosclerosis in diabetes: pathogenic and therapeutic implications.

Authors:  Margo P Cohen; Fuad N Ziyadeh; Sheldon Chen
Journal:  J Lab Clin Med       Date:  2006-05

4.  Macrophage colony-stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro.

Authors:  Wei Liu; Ge Z Xu; Chun H Jiang; Jie Tian
Journal:  BMC Cell Biol       Date:  2011-01-25       Impact factor: 4.241

5.  Magnetic techniques for the isolation and purification of proteins and peptides.

Authors:  Ivo Safarik; Mirka Safarikova
Journal:  Biomagn Res Technol       Date:  2004-11-26
  5 in total

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